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首页> 外文期刊>Applied Microbiology >Adhesive Bond Stiffness of Staphylococcus aureus with and without Proteins That Bind to an Adsorbed Fibronectin Film
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Adhesive Bond Stiffness of Staphylococcus aureus with and without Proteins That Bind to an Adsorbed Fibronectin Film

机译：有和没有结合到吸附的纤连蛋白膜上的蛋白的金黄色葡萄球菌的粘合键刚度

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摘要

Staphylococcus aureus is known to cause biomaterial-associated infections of implants and devices once it has breached the skin and mucosal barriers. Adhesion is the initial step in the development of a biomaterial-associated infection, and strategies to prevent staphylococcal adhesion and thus biomaterial-associated infections require understanding of the adhesive bond. The aim of this study was to compare the adhesive bond stiffnesses of two S. aureus strains with and without fibronectin-binding proteins (FnBPs) adhering to a fibronectin-coated quartz crystal microbalance (QCM) sensor surface on the basis of a coupled- resonance model. Both fibronectin adsorption and staphylococcal adhesion were accompanied by negative frequency shifts, regardless of the absence or presence of FnBPs on the staphylococcal cell surfaces. This is the opposite of the positive frequency shifts often observed for other bacterial strains adhering to bare sensor surfaces. Most likely, adhering staphylococci sink into and deform the adsorbed protein layer, creating stiff binding with the sensor surface due to an increased bacterium-substratum contact area. S. aureus 8325-4 possesses FnBPs and yields less negative frequency shifts (Δ f ) that are further away from the zero-crossing frequency than S. aureus DU5883. This suggests that FnBPs on S. aureus 8325-4 create a stiffer bond to the fibronectin coating than has been observed for S. aureus DU5883. Due to a limited window of observation, as defined by the available resonance frequencies in QCM, we could not determine exact stiffness values.
机译：已知金黄色葡萄球菌突破皮肤和粘膜屏障后，会引起生物材料相关的植入物和器械感染。粘附是生物材料相关感染发展的第一步，而预防葡萄球菌粘附并因此预防生物材料相关感染的策略需要了解粘附键。这项研究的目的是在耦合共振的基础上，比较两种带有和不带有纤连蛋白结合蛋白（FnBP）的金黄色葡萄球菌菌株粘附在纤连蛋白涂层的石英晶体微天平（QCM）传感器表面上的粘附力。模型。纤连蛋白吸附和葡萄球菌粘附均伴随负频移，而不管葡萄球菌细胞表面上是否存在FnBP。这与粘附在裸露传感器表面上的其他细菌菌株经常观察到的正频移相反。最有可能的是，由于增加的细菌-基质接触面积，粘附的葡萄球菌会沉入吸附的蛋白质层并使之变形，从而与传感器表面形成牢固的结合。金黄色葡萄球菌8325-4具有FnBP，并且产生比金黄色葡萄球菌DU5883更少的负频移（Δf），其更远离零交叉频率。这表明，金黄色葡萄球菌8325-4上的FnBP与纤连蛋白涂层形成的结合比金黄色葡萄球菌DU5883更坚硬。由于有限的观察窗口（由QCM中可用的共振频率定义），我们无法确定确切的刚度值。

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