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首页> 外文期刊>Applied Microbiology >Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells
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Functional Analysis of Lactobacillus rhamnosus GG Pili in Relation to Adhesion and Immunomodulatory Interactions with Intestinal Epithelial Cells

机译：鼠李糖乳杆菌GG Pili的功能分析与肠上皮细胞的粘附和免疫调节相互作用

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Lactobacillus rhamnosus GG, a probiotic with good survival capacity in the human gut, has well-documented adhesion properties and health effects. Recently, spaCBA -encoded pili that bind to human intestinal mucus were identified on its cell surface. Here, we report on the phenotypic analysis of a spaCBA pilus knockout mutant in comparison with the wild type and other adhesin mutants. The SpaCBA pilus of L. rhamnosus GG showed to be key for efficient adherence to the Caco-2 intestinal epithelial cell (IEC) line and biofilm formation. Moreover, the spaCBA mutant induces an elevated level of interleukin-8 (IL-8) mRNA in Caco-2 cells compared to the wild type, possibly involving an interaction of lipoteichoic acid with Toll-like receptor 2. In contrast, an L. rhamnosus GG mutant without exopolysaccharides but with an increased exposure of pili leads to the reduced expression of IL-8. Using Transwells to partition bacteria from Caco-2 cells, IL-8 induction is blocked completely regardless of whether wild-type or mutant L. rhamnosus GG cells are used. Taken together, our data suggest that L. rhamnosus GG SpaCBA pili, while promoting strong adhesive interactions with IECs, have a functional role in balancing IL-8 mRNA expression induced by surface molecules such as lipoteichoic acid.
机译：鼠李糖乳杆菌GG是一种益生菌，在人的肠道中具有良好的生存能力，具有充分证明的粘附特性和健康影响。最近，在其细胞表面鉴定了结合人肠粘液的spaCBA编码菌毛。在这里，我们报告了与野生型和其他粘附素突变体相比，spaCBA菌毛敲除突变体的表型分析。鼠李糖乳杆菌GG的SpaCBA菌毛显示出对Caco-2肠上皮细胞（IEC）系和生物膜形成的有效粘附的关键。此外，与野生型相比，spaCBA突变体在Caco-2细胞中诱导了较高水平的白介素8（IL-8）mRNA表达，这可能涉及脂磷壁酸与Toll样受体2的相互作用。没有胞外多糖但菌毛暴露增加的鼠李糖GG突变体导致IL-8表达降低。使用Transwells将细菌从Caco-2细胞中分离出来，无论使用野生型还是突变型鼠李糖乳杆菌GG细胞，均可完全阻断IL-8诱导。两者合计，我们的数据表明，鼠李糖乳杆菌GG SpaCBA菌毛，虽然促进与IECs的强粘性相互作用，但在平衡由表面脂蛋白等表面分子诱导的IL-8 mRNA表达方面具有功能性作用。

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