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Deletion Mutant Library for Investigation of Functional Outputs of Cyclic Diguanylate Metabolism in Pseudomonas aeruginosa PA14

机译:缺失突变体文库,用于研究铜绿假单胞菌PA14中循环双鸟苷酸代谢的功能输出

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We constructed a library of in-frame deletion mutants targeting each gene in Pseudomonas aeruginosa PA14 predicted to participate in cyclic di-GMP (c-di-GMP) metabolism (biosynthesis or degradation) to provide a toolkit to assist investigators studying c-di-GMP-mediated regulation by this microbe. We present phenotypic assessments of each mutant, including biofilm formation, exopolysaccharide (EPS) production, swimming motility, swarming motility, and twitch motility, as a means to initially characterize these mutants and to demonstrate the potential utility of this library.
机译:我们构建了一个针对特定铜绿假单胞菌PA14中每个基因的框内缺失突变体文库,这些突变体预计将参与循环双GMP(c-di-GMP)代谢(生物合成或降解),以提供一个工具包来协助研究者研究c-di- GMP介导的微生物调控。我们介绍了每个突变体的表型评估,包括生物膜形成,胞外多糖(EPS)产生,游泳运动,成群运动和抽搐运动,以此作为初始表征这些突变体和证明该文库潜在用途的手段。

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