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首页> 外文期刊>Applied Microbiology >Clearance of Escherichia coli O157:H7 Infection in Calves by Rectal Administration of Bovine Lactoferrin
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Clearance of Escherichia coli O157:H7 Infection in Calves by Rectal Administration of Bovine Lactoferrin

机译：牛乳铁蛋白的直肠给药可清除犊牛中的大肠杆菌O157：H7感染

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Enterohemorrhagic Escherichia coli (EHEC) strains, of which E. coli O157:H7 is the best-studied serotype, are an important group of foodborne pathogens causing severe illness in humans worldwide. The main reservoirs for EHEC are ruminants, mostly cattle, which harbor the bacteria in their intestinal tracts without showing clinical symptoms. In this study, we used bovine lactoferrin, a natural occurring bactericidal and immunomodulating protein, as an antibacterial agent against EHEC infection in cattle. Nine 3-month-old Holstein-Friesian calves were experimentally infected with EHEC (strain NCTC12900). Three animals received a daily rectal spray treatment with bovine lactoferrin, three animals received an oral treatment, and three animals served as a control group. Blood samples were collected weekly and fecal samples twice weekly to monitor antibody responses and fecal excretion, respectively. Animals in the rectal group ceased shedding within 26 days of the experimental treatment and remained negative. This beneficial effect of bovine lactoferrin was not observed in the oral group, where animals were still shedding at the time of euthanasia (day 61). All groups developed serum responses, but no clear differences could be observed between the groups. However, the results indicate that the use of bovine lactoferrin as a rectal treatment can be a useful strategy to preclude further transmission of EHEC infections from cattle to humans.
机译：肠出血性大肠杆菌（EHEC）菌株，其中O157：H7大肠杆菌是研究最多的血清型，是导致全世界人类严重疾病的重要食源性病原体。 EHEC的主要储藏体是反刍动物，大多数是牛，它们在肠道内藏有细菌而没有临床症状。在这项研究中，我们使用了牛乳铁蛋白（一种天然的杀菌和免疫调节蛋白）作为针对牛EHEC感染的抗菌剂。对9个3个月大的Holstein-Friesian小牛进行了实验性感染EHEC（菌株NCTC12900）。每天对三只动物进行牛乳铁蛋白的直肠喷雾治疗，三只动物进行口服治疗，三只动物作为对照组。每周采集一次血液样品，每周两次采集粪便样品，以分别监测抗体反应和粪便排泄。直肠组的动物在实验治疗后的26天内停止脱落，并保持阴性。在口服组中未观察到牛乳铁蛋白的这种有益作用，在安乐死时（第61天）动物仍在脱落。所有组均出现血清反应，但各组之间未观察到明显差异。但是，结果表明，使用牛乳铁蛋白作为直肠治疗可能是防止EHEC感染从牛进一步传播给人类的有用策略。

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