• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IFAC PapersOnLine >Metabolic modeling of C. sorokiniana diauxic heterotrophic growth
【24h】

Metabolic modeling of C. sorokiniana diauxic heterotrophic growth

机译： C的代谢建模。 sorokiniana 双营养异养生长

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Abstract: Microalgae are promising microorganisms for the production of numerous molecules of interest, such as pigments, proteins or triglycerides that can be turned into biofuels. Heterotrophic growth on wastes represents an interesting approach to achieve higher biomass concentrations, while reducing cost and improving the environmental footprint. Wastes generally consist of a mixt of diverse molecules. It is crucial to understand microalgal metabolism in such conditions, where switching between substrates might occur. Metabolic modeling has proven to be an efficient tool for understanding metabolism and guiding the optimization of biomass or target molecule production. Here, we focused on the metabolism of Chlorella sorokiniana growing heterotrophically on acetate and butyrate. The metabolism was represented by 163 metabolic reactions. The DRUM modeling framework, with a mildly relaxed quasi-steady-state assumption, was used to account for possible intracellular accumulation during switching between substrates. Six experiments were used to calibrate the model and eight experiments for the validation. The model efficiently predicted the experimental data, including the transient behavior. To the best of our knowledge, this is the first study to describe the dynamic metabolic fluxes of microalgae during heterotrophic and diauxic growth. It shows that an accurate model of metabolism can now be constructed, even in dynamic conditions, with the presence of several carbon substrates. It also opens new perspectives for the heterotrophic use of microalgae, especially for biofuel production from wastes.
机译：摘要：微藻是一种有前途的微生物，可用于生产多种目标分子，例如可以转化为生物燃料的色素，蛋白质或甘油三酸酯。废物的异养生长是实现更高的生物质浓度，同时降低成本和改善环境足迹的有趣方法。废物通常由各种分子的混合物组成。了解这种情况下可能发生底物之间切换的微藻代谢至关重要。代谢建模已被证明是了解代谢并指导生物质或目标分子生产的优化的有效工具。在这里，我们专注于在乙酸盐和丁酸盐上异养生长的小球藻的代谢。代谢以163个代谢反应为代表。 DRUM建模框架带有一个轻度宽松的准稳态假设，用于解释在底物之间切换期间可能的细胞内积累。六个实验用于校准模型，八个实验用于验证。该模型有效地预测了实验数据，包括瞬态行为。据我们所知，这是第一个描述异养和双生生长过程中微藻动态代谢通量的研究。它表明，即使在动态条件下，存在多种碳底物，也可以构建精确的代谢模型。它也为微藻的异养用途，特别是利用废物生产生物燃料开辟了新的前景。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号