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Quantitative Chemical Composition of Peptidoglycan of Listeria monocytogenes

机译:李斯特菌李斯特菌肽聚糖的定量化学组成

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The qualitative and quantitative chemical composition of the peptidoglycan layer of Listeria monocytogenes cell walls was determined. The peptidoglycan layer was isolated from intact cells after treatment with sodium lauryl sulfate and digestion with Pronase. The isolated peptidoglycan contained carbohydrates which included hexose, rhamnose, and amino sugars. The amino sugar was identified as glucosamine which was present in very high micromole concentration, as compared with the amino acids, muramic acid, or diaminopimelic acid. In addition, protein and phosphorus were also present. However, the presence of teichoic acid was not detected in the peptidoglycan. Protein moiety contained a total of 18 amino acids with lysine as the N-terminal amino acid.
机译:测定了单核细胞增生李斯特菌细胞壁肽聚糖层的定性和定量化学组成。用月桂基硫酸钠处理并用Pronase消化后,从完整细胞中分离出肽聚糖层。分离的肽聚糖包含碳水化合物,包括己糖,鼠李糖和氨基糖。与氨基酸,山as酸或二氨基庚二酸相比,氨基糖被鉴定为以非常高的微摩尔浓度存在的葡糖胺。另外,蛋白质和磷也存在。但是,在肽聚糖中未检测到硫磷酸的存在。蛋白质部分总共包含18个氨基酸,赖氨酸为N末端氨基酸。

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