• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Effects of Trypan Blue Treatment on the Immune Responses of Mice
【24h】

Effects of Trypan Blue Treatment on the Immune Responses of Mice

机译：锥虫蓝处理对小鼠免疫反应的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

It has been reported that trypan blue treatment decreases the nonspecific resistance of mice to transplanted tumors and inhibits the in vitro cytotoxic activity of activated macrophages. We wished to determine whether this effect of trypan blue could be due to a selective inhibition of certain macrophage functions or whether it reflected a broader form of immunosuppression. We therefore tested the effects of trypan blue on a variety of immunological responses. Treatment of mice with trypan blue delayed their rejection of skin allografts and transplants of a highly antigenic syngeneic ultraviolet light-induced tumor. Trypan blue treatment of either donor or recipient decreased the local graft-versus-host reaction. Filtration of lymph node cells from trypan blue-treated donors on a nylon wool column before use in the graft-versus-host assay abrogated the depressive effect of trypan blue. A transient reduction in the blastogenic response of spleen cells to concanavalin A and lipopolysaccharide mitogens was observed after a single injection of trypan blue, but the response of lymph node cells was unaffected. The depressed response of splenic lymphocytes was not entirely reversed by removal of adherent cells. The primary and secondary hemagglutinin responses to sheep erythrocytes were unaffected in trypan blue-treated mice, and the proportion and phagocytic activity of thioglycolate-induced peritoneal macrophages were also unaltered. We conclude that treatment of mice with trypan blue selectively inhibits certain macrophage functions but, at high doses, it can also inhibit some lymphocyte activities.
机译：据报道，锥虫蓝治疗降低了小鼠对移植肿瘤的非特异性抗性，并抑制了活化巨噬细胞的体外细胞毒活性。我们希望确定锥虫蓝的这种作用是否可能是由于某些巨噬细胞功能的选择性抑制或是否反映了更广泛的免疫抑制形式。因此，我们测试了锥虫蓝对多种免疫反应的影响。用台盼蓝治疗小鼠可延缓其皮肤同种异体移植和高度抗原性同基因紫外线诱导的肿瘤移植的排斥。锥虫蓝对供体或受体的治疗均降低了局部移植物抗宿主反应。在用于移植物抗宿主试验之前，在尼龙羊毛柱上过滤了经锥虫蓝处理的供体的淋巴结细胞，从而消除了锥虫蓝的抑制作用。单次注射锥虫蓝后，观察到脾细胞对伴刀豆球蛋白A和脂多糖有丝分裂原的胚细胞反应的瞬时减少，但淋巴结细胞的反应未受影响。脾脏淋巴细胞的抑郁反应并未通过去除粘附细胞而完全逆转。锥虫蓝处理的小鼠对绵羊红细胞的初级和次级血凝素反应不受影响，巯基乙酸盐诱导的腹膜巨噬细胞的比例和吞噬活性也未改变。我们得出的结论是，用锥虫蓝对小鼠的治疗选择性地抑制了某些巨噬细胞的功能，但是在高剂量时，它也可以抑制某些淋巴细胞的活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号