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首页> 外文期刊>Infection and immunity >Inhibition of the growth of Neisseria meningitidis by reduced ferritin and other iron-binding agents.
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Inhibition of the growth of Neisseria meningitidis by reduced ferritin and other iron-binding agents.

机译：铁蛋白减少和其他铁结合剂抑制脑膜炎奈瑟氏球菌的生长。

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Serogroups of N. meningitidis were characterized as virulent or avirulent according to their capacity to establish meningococcal infection in mice. An agar plate diffusion technique demonstrated that iron had a definite growth-supporting role for both of these meningococcal types. The avirulent strains could use ionic or chelated iron as well as the virulent strains. Iron-reversible growth inhibition occurred to the same extent for both bacterial types in the presence of the synthetic iron-chelating agents Desferal and ethylenediamine-di-orthohydroxy phenylacetic acid. A difference in response was demonstrated for these bacterial types when grown in the presence of various iron-binding proteins from animal body fluids and tissues. The growth of the avirulent strain was inhibited to a greater degree by egg white conalbumin. The humoral iron-binding protein transferrin showed a significant inhibitory capacity only when used in conjunction with bicarbonate. Under conditions of increased iron saturation of this protein, the avirulent strain was inhibited to the furthest extent. In the presence of ferritin, the cellular iron-binding protein, which had been reduced, inhibition of the growth of either strain type did not occur on iron-poor media (less than 5 micrograms/100 ml). However, with the incorporation of iron into the media, the inhibitory effect of the protein became evident. As the concentration of iron increased, the inhibition increased to a certain level and subsequently declined. A substantial difference in the ability of the avirulent type to grow in the presence of reduced horse spleen ferritin was observed. For this microorganism, a correlation appears to exist between the capacity to grow by utilizing the available iron in the presence of reduced ferritin and the ability to establish infection. The host protein ferritin, in the reduced state, apart from simply being a storage protein for iron, can prevent the growth of a procaryotic organism. Our experiments suggest a role for ferritin in the prevention of emningococcal disease. A cehmotherapeutic potential for Desferal is also implied.
机译：脑膜炎奈瑟氏球菌的血清群根据其在小鼠中建立脑膜炎球菌感染的能力被鉴定为有毒或无毒。琼脂平板扩散技术表明，铁对于这两种脑膜炎球菌都有明确的生长支持作用。无毒力菌株可以使用离子或螯合铁以及强毒力菌株。在合成铁螯合剂Desferal和乙二胺-二邻羟基苯乙酸的存在下，两种细菌对铁的可逆生长抑制程度均相同。当在各种来自动物体液和组织的铁结合蛋白的存在下生长时，这些细菌类型的反应差异得到了证明。无蛋白菌株的生长被蛋清伴白蛋白更大程度地抑制。仅当与碳酸氢盐一起使用时，体液结合铁的蛋白转铁蛋白才显示出显着的抑制能力。在该蛋白质的铁饱和度增加的条件下，无毒菌株被最大程度地抑制。在铁蛋白减少的细胞铁结合蛋白存在的情况下，在铁缺乏的培养基（少于5微克/ 100 ml）上，不会出现对任一菌株类型生长的抑制作用。然而，随着铁掺入培养基中，蛋白质的抑制作用变得明显。随着铁浓度的增加，抑制作用增加到一定水平，然后下降。观察到无毒性类型在马脾铁蛋白减少的情况下生长的能力存在显着差异。对于这种微生物，似乎在铁蛋白还原的情况下利用可利用的铁的生长能力与建立感染的能力之间存在相关性。处于还原状态的宿主蛋白铁蛋白不仅是铁的贮藏蛋白，还可以防止原核生物的生长。我们的实验表明铁蛋白在预防球菌病中的作用。还暗示了Desferal的脑电疗法的潜力。

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