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首页> 外文期刊>Infection and immunity >Adhesion of Aeromonas salmonicida strains associated with net electrostatic charges of host tissue cells.
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Adhesion of Aeromonas salmonicida strains associated with net electrostatic charges of host tissue cells.

机译：鲑鱼气单胞菌菌株的粘附与宿主组织细胞的净静电荷有关。

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The adhesion of Aeromonas salmonicida, the pathogenic bacterium of fish furunculosis in salmon and trout, to the surface of host tissue cells was investigated with two fish tissue culture cell lines (RTG-2 cells from rainbow trout, Salmo gairdneri, and CHSE-214 cells from chinook salmon, Oncorhynchus tshawytscha) and four A. salmonicida strains. Bacterial cells of pathogenic strains were highly adhesive to RTG-2 and CHSE-214 cells and were negatively charged in the net electrostatic charges, as determined by electrophoresis on filter paper strips at pH 7, whereas bacterial cells of nonpathogenic strains were nonadhesive and positively charged. The electrophoresis of RTG-2 and CHSE-214 cells with balanced salt solution (BSS), phosphate-buffered saline, or fish serum diluted with BSS (pH 7) was carried out with an appropriate electrophoretic apparatus that was devised for this study. After electrophoresis with 20 mA of direct current for 15 min at pH 7, the electrophoretic dispositions of these tissue culture cells were determined by the mode of frequency of occurrence of these cells in the partitioned chambers of the device. RTG-2 and CHSE-214 cells with BSS and fish serum were attracted from the central chamber (to which each cell sample was added) to the cathode chambers, but no attraction was detected when these cells were used with phosphate-buffered saline. Noradrenaline- and phosphoenolpyruvate-pretreated RTG-2 cells migrated more to the cathode chambers, whereas succinate- and valine-pretreated RTG-2 cells moved to the anode chambers. These movements to the cathode and anode were alleviated by the use of RTG-2 cells preincubated with pathogenic and nonpathogenic bacterial cells, respectively. The adhesion of the pathogenic bacteria to RTG-2 cells was enhanced by the use of RTG-2 cells pretreated with noradrenaline and phosphoenolpyruvate, whereas the nonpathogenic bacteria were adherent to RTG-2 cells pretreated with succinate and valine. These findings indicate that the adhesion of A. salmonicida strains to host tissue cells is closely associated with mutually converse net electrostatic charges.
机译：用两种鱼类组织培养细胞系（虹鳟鱼的RTG-2细胞，Salmo gairdneri和CHSE-214细胞）研究了鲑鱼和鳟鱼中的鱼糠un病的致病菌鲑鱼气单胞菌对宿主组织细胞的粘附。来自奇努克鲑鱼，Oncorhynchus tshawytscha）和四种沙门氏菌菌株。致病菌株的细菌细胞与RTG-2和CHSE-214细胞具有很高的粘附性，并且在净静电荷中带负电荷，这是通过在pH 7的滤纸条上进行电泳确定的，而非致病性菌株的细菌细胞是非粘附性的且带正电荷的。 RTG-2和CHSE-214细胞用平衡盐溶液（BSS），磷酸盐缓冲盐水或BSS稀释的鱼血清（pH 7）进行电泳，这是为该研究设计的一种合适的电泳仪。在pH值为7的20 mA直流电下电泳15分钟后，这些组织培养细胞的电泳布置取决于这些细胞在设备分隔室中的出现频率。具有BSS和鱼血清的RTG-2和CHSE-214细胞从中央室（向其中添加了每个细胞样品）被吸引到阴极室，但是当这些细胞与磷酸盐缓冲盐水一起使用时，没有检测到吸引力。去甲肾上腺素和磷酸烯醇丙酮酸预处理的RTG-2细胞更多地迁移到阴极室，而琥珀酸酯和缬氨酸预处理的RTG-2细胞移动到阳极室。通过分别与病原性和非病原性细菌细胞预孵育的RTG-2细胞，可减轻向阴极和阳极的这些移动。通过使用去甲肾上腺素和磷酸烯醇丙酮酸预处理的RTG-2细胞可以增强致病菌对RTG-2细胞的粘附，而非致病菌则可以与琥珀酸和缬氨酸预处理的RTG-2细胞粘附。这些发现表明，沙门氏菌菌株对宿主组织细胞的粘附与相互相反的净静电荷密切相关。

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