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首页> 外文期刊>Infection and immunity >Isolation and characterization of recombinant antigens from Leishmania aethiopica that react with human antibodies.
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Isolation and characterization of recombinant antigens from Leishmania aethiopica that react with human antibodies.

机译：分离和鉴定与人类抗体反应的埃塞俄比亚利什曼原虫的重组抗原。

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A genomic expression library of Leishmania aethiopica was constructed in lambda gt11 and screened with patient sera and sera from healthy people living in an area of endemicity. Forty-five recombinant clones were isolated and partly characterized. Clone-specific antibodies were prepared and used with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western immunoblot analysis to estimate the molecular masses of the parasite-derived antigens containing the reactive epitope(s). Antigens with apparent molecular masses of 90, 85, 63, 50, 41, 25 and 24 kDa as well as several antigens with lower molecular masses were detected. The clone-specific antibodies from patients with diffuse cutaneous leishmaniasis reacted with high-molecular-weight antigens (30,000 less than Mr less than 90,000), whereas antibodies from patients with localized cutaneous leishmaniasis recognized low-molecular-weight antigens (Mr less than 25,000). Nine different purified recombinant antigens were obtained from lysogens in Escherichia coli Y1089 by immunoaffinity chromatography on anti-beta-galactosidase columns and were subsequently tested with patient sera. It is suggested that some of these recombinant antigens might be used for immunodiagnostic purposes.
机译：在λgt11中构建了埃塞俄比亚利什曼原虫的基因组表达文库，并用患者血清和来自流行地区的健康人群的血清进行了筛选。分离了45个重组克隆并部分表征。制备了克隆特异性抗体，并将其与十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和Western免疫印迹分析一起使用，以估计包含反应性表位的寄生虫衍生抗原的分子量。检测到表观分子量为90、85、63、50、41、25和24 kDa的抗原，以及几种分子量较低的抗原。来自弥漫性皮肤利什曼病的患者的克隆特异性抗体与高分子量抗原发生反应（比小于90,000的Mr少30,000），而来自局部皮肤利什曼病的患者的抗体识别为低分子量抗原（Mr小于25,000）。 。通过抗β-半乳糖苷酶柱上的免疫亲和色谱法从大肠杆菌Y1089的溶菌原中获得了九种不同的纯化重组抗原，随后用患者血清进行了检测。建议这些重组抗原中的一些可以用于免疫诊断目的。

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