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Attachment of Cryptosporidium parvumSporozoites to Human Intestinal Epithelial Cells

机译:小隐孢子虫子孢子对人肠上皮细胞的附着

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An enzyme-linked immunosorbent assay-based attachment model using the human intestinal cell line Caco-2A was developed to study attachment of Cryptosporidium parvum sporozoites in vitro and to assess potential inhibitors of sporozoite binding. In this system, attachment was related to sporozoite dose, incubation time, and host cell differentiation status. Polyclonal antibodies to C. parvum as well as glycoprotein inhibitors of a sporozoite lectin reduced attachment. This model will be a valuable tool in elucidating specific molecules and mechanisms involved in sporozoite-host cell attachment.
机译:建立了一种基于酶联免疫吸附测定的基于人肠道细胞系Caco-2A的附着模型,以研究 Cryptosporidium parvum 子孢子的体外附着力,并评估潜在的子孢子结合抑制剂。在该系统中,附着与子孢子的剂量,孵育时间和宿主细胞的分化状态有关。针对 C的多克隆抗体。小孢子以及子孢子凝集素的糖蛋白抑制剂可减少附着。该模型将是阐明与子孢子-宿主细胞附着有关的特定分子和机制的有价值的工具。

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