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首页> 外文期刊>Infection and immunity >Parachlamydia acanthamoeba Enters and Multiplies within Human Macrophages and Induces Their Apoptosis
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Parachlamydia acanthamoeba Enters and Multiplies within Human Macrophages and Induces Their Apoptosis

机译：衣原体棘皮动物进入并在人类巨噬细胞中繁殖并诱导其凋亡

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Parachlamydia acanthamoeba is an obligately intracellular bacterium that naturally infects free-living amoebae. It is a potential human pathogen and may survive in human macrophages. We studied P. acanthamoeba entry into, and multiplication within, human monocyte-derived macrophages. After 8 h of incubation, 80% of macrophages were infected with a mean of 3.8 P. acanthamoeba organisms per cell. Electron microscopy demonstrated that parachlamydiae were in an intracellular vacuole. After infection with living organisms, the number of parachlamydiae per macrophage increased 4 times from day 0 to day 4, whereas heat-inactivated parachlamydiae were eliminated during the same period. Quantitative PCR confirmed that P. acanthamoeba replicates within macrophages. Transcriptional activity of P. acanthamoeba was detected by reverse transcription-PCR targeting the gene encoding ADP-ATP translocase (tlc). P. acanthamoeba exerted a cytopathic effect on macrophages. When macrophages were infected with living bacteria, their number decreased significantly from day 0 to day 4 due to apoptosis, as shown by annexin-V binding and electron microscopy. This study shows that P. acanthamoeba enters and multiplies within human macrophages before inducing their apoptosis.
机译：肺炎衣原体是一种专性的细胞内细菌，可自然感染自由活动的变形虫。它是潜在的人类病原体，可以在人类巨噬细胞中存活。我们研究了 P。棘阿米巴进入人单核细胞衍生的巨噬细胞并在其中繁殖。温育8小时后，感染80％的巨噬细胞，平均为3.8P。每个细胞的棘阿米巴生物。电子显微镜显示衣原体位于细胞内液泡中。在感染了活生物体后，从第0天到第4天，每个巨噬细胞的衣原体数量增加了4倍，而同期热灭活的衣原体则被消除了。定量PCR证实了 P。棘阿米巴在巨噬细胞内复制。 P的转录活性。逆转录-PCR法检测acanthamoeba ，该基因靶向ADP-ATP转位酶（ tlc ）编码的基因。 P。棘阿米巴对巨噬细胞具有细胞病变作用。如膜联蛋白-V结合和电子显微镜观察，当巨噬细胞被活细菌感染时，由于凋亡，它们的数目从第0天到第4天显着下降。这项研究表明 P。棘阿米巴进入并在人类巨噬细胞内繁殖，然后诱导其凋亡。

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