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首页> 外文期刊>Infection and immunity >Binding kinetics of Clostridium difficile toxins A and B to intestinal brush border membranes from infant and adult hamsters.
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Binding kinetics of Clostridium difficile toxins A and B to intestinal brush border membranes from infant and adult hamsters.

机译：艰难梭菌毒素A和B与婴儿和成年仓鼠肠道刷状缘膜的结合动力学。

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This study was undertaken to determine if the relative resistance of neonates and infants to Clostridium difficile-associated intestinal disease can be related to age-dependent differences in intestinal receptors for C. difficile toxins A and B. Brush border membranes (BBMs) from the small intestines of adult and infant hamsters were examined for their ability to bind radiolabeled toxins A and B. [125I]toxin A bound to both infant and adult hamster BBMs at physiological temperature, whereas [125I]toxin B did not bind to the BBMs under any of the conditions examined. The number of [125I]toxin A molecules bound at saturation was approximately 4 x 10(10) per micrograms of membrane protein for adult BBMs and 1 x 10(11) per micrograms of membrane protein for infant BBMs. Scatchard plot analysis suggested the presence of a single class of toxin A binding sites on both infant and adult hamster BBMs. Maximal binding capacity and Kd values were 0.63 pmol/mg of protein and 66.7 nM, respectively, for the infant BBMs, and 0.24 pmol/mg of protein and 27 nM, respectively, for the adult BBMs. Sodium dodecyl sulfate-polyacrylamide gel electrophoretic analyses of extracted BBM proteins revealed differences in the proteins of infant and adult BBMs. However, there were not any detectable differences in the protein bands which bound [125I]toxin A between infant and adult hamsters. The results from these investigations indicate that differences in the binding kinetics of toxins A and/or B to infant and adult hamster BBMs do not account for the observed differences in their susceptibility to C. difficile-associated intestinal disease.
机译：进行这项研究是为了确定新生儿和婴儿对艰难梭菌相关肠道疾病的相对耐药性是否可能与艰难梭菌毒素A和B的肠道受体的年龄依赖性差异有关。从较小的刷状缘膜（BBM）检查了成年和婴儿仓鼠的肠道结合放射标记毒素A和B的能力。[125I]毒素A在生理温度下与婴儿和成年仓鼠BBM都结合，而[125I]毒素B在任何温度下均不与BBM结合。检查的条件。对于成人BBM，饱和结合的[125I]毒素A分子的数量约为每微克膜蛋白4 x 10（10），对于婴儿BBM约为每微克膜蛋白1 x 10（11）。斯卡查德图分析表明，在婴儿和成年仓鼠BBM上都存在一类毒素A结合位点。婴儿BBMs的最大结合能力和Kd值分别为0.63 pmol / mg蛋白和66.7 nM，成人BBMs的最大结合能力和Kd值分别为0.24 pmol / mg蛋白和27 nM。提取的BBM蛋白的十二烷基硫酸钠-聚丙烯酰胺钠凝胶电泳分析显示，婴儿和成人BBMs的蛋白质存在差异。但是，在婴儿和成年仓鼠之间结合[125I]毒素A的蛋白条带上没有发现任何可检测的差异。这些研究的结果表明，毒素A和/或B与婴儿和成年仓鼠BBM的结合动力学差异并未解释观察到的它们对艰难梭菌相关肠道疾病的敏感性。

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