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首页> 外文期刊>Infection and immunity >Mouse liver contains a Pseudomonas aeruginosa exotoxin A-binding protein.
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Mouse liver contains a Pseudomonas aeruginosa exotoxin A-binding protein.

机译：小鼠肝脏中含有铜绿假单胞菌外毒素A结合蛋白。

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The opportunistic pathogen Pseudomonas aeruginosa produces several potential virulence factors, including the ADP-ribosylating toxin, exotoxin A (PE). Studies using a burned mouse model have shown that PE consistently inhibits protein synthesis and depletes elongation factor 2 in mouse liver and variably in other organs. One reason for toxin sensitivity could be the presence of a PE receptor on the surface of cells. Therefore we examined detergent extracts of mouse tissues for the presence of toxin-binding proteins. Proteins which specifically bind PE were present in extracts from liver, kidney, lung, spleen, and heart. Because liver appears to be a prominent target for the toxin in a burned animal, we choose to isolate the PE-binding protein from mouse liver and compare this protein to the recently characterized toxin-binding protein from toxin-sensitive mouse LM fibroblasts. The toxin-binding proteins from both sources have a molecular mass of approximately 350 kDa, share similar protease digestion profiles, and are glycosylated. However the glycosylation patterns for the two species are quite different. Both glycoproteins bind toxin with high avidity. The toxin-binding moiety is located, at least in part, on the plasma membrane and thus could represent the receptor involved in internalization of toxin molecules responsible for cell death.
机译：机会性病原体铜绿假单胞菌产生几种潜在的毒力因子，包括ADP-核糖基化毒素，外毒素A（PE）。使用烧伤的小鼠模型的研究表明，PE始终抑制蛋白质合成，并耗尽小鼠肝脏以及其他器官中的可变伸长因子2。毒素敏感性的一个原因可能是细胞表面存在PE受体。因此，我们检查了小鼠组织去污剂提取物中毒素结合蛋白的存在。特异性结合PE的蛋白质存在于肝，肾，肺，脾和心脏的提取物中。由于肝脏似乎是烧伤动物中毒素的主要靶标，因此我们选择从小鼠肝脏中分离PE结合蛋白，并将该蛋白与最近对毒素敏感的小鼠LM成纤维细胞表征的毒素结合蛋白进行比较。来自两种来源的毒素结合蛋白的分子量约为350 kDa，具有相似的蛋白酶消化谱，并且被糖基化。但是，这两个物种的糖基化模式完全不同。两种糖蛋白均以高亲和力结合毒素。毒素结合部分至少部分位于质膜上，因此可以代表参与负责细胞死亡的毒素分子内在化的受体。

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