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Accessory Gene Regulator Locus of Staphylococcus intermedius

机译：中间葡萄球菌的辅助基因调控基因座

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The accessory gene regulator (agr) locus, a candidate system for the regulation of the production of virulence factors in Staphylococcus intermedius, has been characterized. Using PCR-based genome walking, we have obtained the first complete sequence (3,436 bp) of the accessory gene regulator (agr) gene in this organism. Sequence analysis of the agr gene has identified five open reading frames (ORFs), agrB, agrD, agrC, agrA, and hld. The translated ORF contained amino acid motifs characteristic of the response regulator and histidine protein kinase signal transducer of the classic two-component regulatory system. Sequencing of the agrD PCR products amplified from DNA from 20 different isolates has facilitated detection of genetic variation in the putative autoinducing peptide (AIP) within the agr gene of S. intermedius, revealing the presence of at least three agr specificity groups within this species. Classification of the agr gene from S. intermedius was supported by phylogenetic analysis. Real-time PCR also revealed that the effector molecule of the agr system, RNAIII, was regulated in an autocrine manner in S. intermedius and demonstrated positive correlation with the temporal gene expression patterns of luk and entC. Transcription of RNAIII was also dependent on self secreted cues. Cyclic self and nonself peptides were synthesized on the basis of the novel AIPs produced by S. intermedius, which lack the cysteine necessary to form the thiolactone ring in analogous peptides from Staphylococcus aureus and Staphylococcus epidermidis. Experiments with these synthetic cyclic peptides indicated that self peptides led to up-regulation of RNAIII—findings in support of the assumption that activation of the agr gene is initiated by growth- and species-specific factors generated during bacterial growth.
机译：辅助基因调节子（ agr ）基因座是调节中间型葡萄球菌（Staphylococcus intermedius）中毒力因子产生的候选系统。使用基于PCR的基因组步移，我们获得了该生物中辅助基因调节子（ agr ）基因的第一个完整序列（3,436 bp）。 agr 基因的序列分析已鉴定出五个开放阅读框（ORF）， agrB ， agrD ， agrC ， agrA 和 hld 。翻译的ORF包含典型的两组分调节系统的响应调节剂和组氨酸蛋白激酶信号转导子的特征性氨基酸基序。从20种不同分离株的DNA中扩增出的 agrD PCR产物的测序有助于检测 S的 agr 基因中推定的自诱导肽（AIP）的遗传变异。 。 intermedius ，揭示了该物种中至少存在三个 agr 特异性基团。 S 中 agr 基因的分类。系统发育分析支持 intermedius 。实时PCR还显示， agr 系统的效应分子RNAIII在 S中以自分泌方式受到调控。 interemius 并与 luk 和 entC 的时间基因表达模式呈正相关。 RNAIII的转录也依赖于自身分泌的线索。在 S产生的新型AIP的基础上合成了环状自身和非自身肽。 intermedius ，在金黄色葡萄球菌和表皮葡萄球菌的类似肽中缺少形成硫代内酯环所必需的半胱氨酸。用这些合成的环状肽进行的实验表明，自身肽导致RNAIII的上调，这一发现支持 agr 基因的激活是由细菌过程中产生的生长和物种特异性因子引发的假设。增长。

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