...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Infection and immunity >Correlations between Antibody Immune Responses at Different Mucosal Effector Sites Are Controlled by Antigen Type and Dosage
【24h】

Correlations between Antibody Immune Responses at Different Mucosal Effector Sites Are Controlled by Antigen Type and Dosage

机译：抗原类型和剂量可控制不同粘膜效应位点的抗体免疫反应之间的相关性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Monitoring specific secretory immunoglobulin A (IgA) responses in the intestines after mucosal immunization or infection is impeded by the fact that sampling of small intestinal secretions requires invasive methods not feasible for routine diagnostics. Since IgA plasma cells generated after intragastric immunization are known to populate remote mucosal sites as well, secretory IgA responses at other mucosal surfaces may correlate to those in the intestines and could serve as proxy measures for IgA secretion in the gut. To evaluate the practicability of this approach, mice were immunized intragastrically with 0.2, 2, and 20 mg of ovalbumin plus 10 μg of cholera toxin, and the antigen-specific local secretory IgA responses in duodenal, ileal, jejunal, rectal, and vaginal secretions, saliva, urine, and feces, as well as serum IgG and IgA responses were analyzed by enzyme-linked immunosorbent assay. Correlation analysis revealed significant relationships between serum IgG and IgA, urinary IgA, salivary IgA, and secretory IgA in duodenal, jejunal, ileal, and rectal secretions for the 0.2-mg but not for the 20-mg ovalbumin dose. Fecal samples were poor predictors for intestinal antiovalbumin IgA responses, and no correlations could be established for cholera toxin, neither between local anti-cholera toxin levels nor to the antiovalbumin responses. Thus, specific IgA in serum, saliva, or urine can serve as a predictor of the release of specific IgA at intestinal surfaces after intragastric immunization, but the lack of correlations for high ovalbumin doses and for cholera toxin indicates a strong dependency on antigen type and dosage for these relationships.
机译：由于小肠分泌物的取样需要常规诊断不可行的侵入性方法，因此难以监测粘膜免疫或感染后肠道中的特定分泌型免疫球蛋白A（IgA）反应。由于已知在胃内免疫后产生的IgA浆细胞也分布在远端的粘膜部位，因此其他粘膜表面的分泌型IgA反应可能与肠道中的反应有关，可以作为肠道中IgA分泌的替代指标。为了评估该方法的实用性，用0.2、2和20 mg的卵清蛋白加10μg霍乱毒素以及十二指肠，回肠，空肠，直肠和阴道分泌物中的抗原特异性局部分泌型IgA反应在胃内免疫小鼠。通过酶联免疫吸附法分析唾液，尿液和粪便，以及血清IgG和IgA反应。相关性分析显示，在0.2 mg的十二指肠，空肠，回肠和直肠分泌物中，血清IgG和IgA，尿液IgA，唾液IgA和分泌型IgA之间存在显着相关性，而20 mg卵清蛋白剂量则无此相关性。粪便样品对于肠道抗卵清蛋白IgA反应的预测性较差，并且与霍乱毒素的相关性既不存在，既不是局部的抗霍乱毒素水平，也不是抗卵清蛋白反应。因此，血清，唾液或尿液中的特异性IgA可以作为胃内免疫后肠道表面特异性IgA释放的预测指标，但缺乏高卵清蛋白剂量和霍乱毒素的相关性表明对抗原类型和这些关系的剂量。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号