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首页> 外文期刊>Infection and immunity >Immunogenicity of Gonococcal Transferrin Binding Proteins during Natural Infections
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Immunogenicity of Gonococcal Transferrin Binding Proteins during Natural Infections

机译：淋球菌转铁蛋白结合蛋白在自然感染过程中的免疫原性。

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In this study, we examined the immune response during gonococcal infection to the individual transferrin binding proteins by using a quantitative enzyme-linked immunosorbent assay (ELISA). Recombinant transferrin binding protein A (rTbpA) and rTbpB were purified under nondenaturing conditions for use as ELISA antigens. Sera and secretions from culture-positive individuals were analyzed for antibodies to rTbpA and rTbpB and compared to samples from individuals with no history of gonococcal infection. Although antibodies to both rTbpA and rTbpB were detected in serum, in most cases the antibody levels were not significantly different from those measured in the control population. Also, previous history of gonococcal infection did not increase antibody levels in serum, suggesting the lack of an anamnestic response. Analysis of secretion samples revealed antibody levels that were generally below the limits of detection in our assay. Overall, this study demonstrated a paucity of systemic and local antibody responses to rTbps as a result of natural infection and represents a baseline over which a protective antibody response will have to be generated in order to develop an efficacious gonococcal vaccine.
机译：在这项研究中，我们使用定量酶联免疫吸附测定（ELISA）检查了淋球菌感染过程中对单个运铁蛋白结合蛋白的免疫反应。在非变性条件下纯化重组转铁蛋白结合蛋白A（rTbpA）和rTbpB，用作ELISA抗原。分析培养阳性患者的血清和分泌物的rTbpA和rTbpB抗体，并与无淋球菌感染史的个体的样品进行比较。尽管在血清中检测到针对rTbpA和rTbpB的抗体，但在大多数情况下，该抗体水平与对照人群中测得的抗体水平没有显着差异。同样，淋球菌感染的既往史也没有增加血清中的抗体水平，提示缺乏记忆消除反应。分泌样品的分析显示抗体水平通常低于我们测定中的检测极限。总体而言，这项研究表明由于自然感染导致对rTbps的全身和局部抗体反应很少，并且代表了基线，为了开发有效的淋球菌疫苗，必须产生保护性抗体反应。

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