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首页> 外文期刊>Infection and immunity >Relapsing Fever Spirochetes Contain Chromosomal Genes with Unique Direct Tandemly Repeated Sequences
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Relapsing Fever Spirochetes Contain Chromosomal Genes with Unique Direct Tandemly Repeated Sequences

机译：复发性发热螺旋体包含具有独特直接串联重复序列的染色体基因

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Genome sequencing of the relapsing fever spirochetes Borrelia hermsii and Borrelia turicatae identified three open reading frames (ORFs) on the chromosomes that contained internal, tandemly repeated amino acid sequences that were absent in the Lyme disease spirochete Borrelia burgdorferi. The predicted amino acid sequences of these genes (BH0209, BH0512, and BH0553) have hydrophobic N termini, indicating that these proteins may be secreted. B. hermsii transcribed the three ORFs in vitro, and the BH0512- and BH0553-encoded proteins (PBH-512 and PBH-553) were produced in vitro and in experimentally infected mice. PBH-512 and PBH-553 were on the spirochete's outer surface, and antiserum to these proteins reduced the adherence of B. hermsii to red blood cells. PCR analyses of 28 isolates of B. hermsii and 8 isolates of B. turicatae demonstrated polymorphism in each gene correlated with the number of repeats. Serum samples from relapsing fever patients reacted with recombinant PBH-512 and PBH-553, suggesting that these proteins are produced during human infection. These polymorphic proteins may be involved in the pathogenicity of these relapsing fever spirochetes and provide a mechanism for antigenic heterogeneity within their populations.
机译：复发性发螺旋体 Borrelia hermsii 和 Borrelia turicatae 的基因组测序在染色体上鉴定出三个开放阅读框（ORF），这些阅读框包含内部串联重复的氨基酸序列，而在莱姆病螺旋体 Borrelia burgdorferi 。这些基因（BH0209，BH0512和BH0553）的预测氨基酸序列具有疏水性N末端，表明这些蛋白质可能被分泌。 B。 hermsii 在体外转录了三个ORF，并在体外和实验感染的小鼠体内产生了BH0512-和BH0553编码的蛋白（PBH-512和PBH-553）。 PBH-512和PBH-553位于螺旋体的外表面，对这些蛋白质的抗血清降低了 B的粘附。疱疹到红细胞。 28株 B的PCR分析。 hermsii 和8个 B分离株。 turicatae 表现出每个基因的多态性与重复次数有关。复发性发热患者的血清样本与重组PBH-512和PBH-553发生反应，表明这些蛋白质是在人类感染期间产生的。这些多态性蛋白可能与这些复发性发热螺旋体的致病性有关，并为其种群内的抗原异质性提供了一种机制。

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