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首页> 外文期刊>Infection and immunity >High-Throughput mRNA Profiling Characterizes the Expression of Inflammatory Molecules in Sepsis Caused by Burkholderia pseudomallei
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High-Throughput mRNA Profiling Characterizes the Expression of Inflammatory Molecules in Sepsis Caused by Burkholderia pseudomallei

机译：高通量mRNA谱表征伯克霍尔德氏菌引起的脓毒症中炎症分子的表达

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摘要

Sepsis is characterized by an uncontrolled inflammatory response to invading microorganisms. We describe the inflammatory mRNA profiles in whole-blood leukocytes, monocytes, and granulocytes using a multigene system for 35 inflammatory markers that included pro- and anti-inflammatory cytokines, chemokines, and signal transduction molecules in a case-control study with 34 patients with sepsis caused by the gram-negative bacterium Burkholderia pseudomallei (the pathogen causing melioidosis) and 32 healthy volunteers. Relative to healthy controls, patients with sepsis showed increased transcription of a whole array of inflammatory genes in peripheral blood leukocytes, granulocytes, and monocytes. Specific monocyte and granulocyte mRNA profiles were identified. Strong correlations were found between inflammatory mRNA expression levels in monocytes and clinical outcome. These data underline the notion that circulating leukocytes are an important source for inflammatory mediators in patients with gram-negative sepsis. Gene profiling such as was done here provides an excellent tool to obtain insight into the extent of inflammation activation in patients with severe infection.
机译：脓毒症的特征在于对入侵微生物的不受控制的炎症反应。在一项针对34例患者的病例对照研究中，我们使用35个炎症标记物（包括促炎和抗炎细胞因子，趋化因子和信号转导分子）的多基因系统，描述了全血白细胞，单核细胞和粒细胞中的炎性mRNA谱。革兰氏阴性细菌<假性伯克霍尔德氏菌（引起类弧菌病的病原体）和32名健康志愿者引起的败血症。相对于健康对照，败血症患者在外周血白细胞，粒细胞和单核细胞中显示出一系列炎症基因的转录增加。鉴定了特定的单核细胞和粒细胞mRNA谱。发现单核细胞中炎性mRNA表达水平与临床结果之间存在强相关性。这些数据强调了循环白细胞是革兰氏阴性败血症患者炎性介质的重要来源的观点。像此处进行的基因分析提供了一个很好的工具，可用来洞察严重感染患者中炎症激活的程度。

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