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首页> 外文期刊>Infection and immunity >Oral Immunization with Escherichia coli Expressing a Lipidated Form of LigA Protects Hamsters against Challenge with Leptospira interrogans Serovar Copenhageni
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Oral Immunization with Escherichia coli Expressing a Lipidated Form of LigA Protects Hamsters against Challenge with Leptospira interrogans Serovar Copenhageni

机译：表达脂质体形式的LigA的大肠杆菌口服免疫保护仓鼠免受问号钩端螺旋体Serovar Copenhageni的攻击

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Leptospirosis is a potentially fatal zoonosis transmitted by reservoir host animals that harbor leptospires in their renal tubules and shed the bacteria in their urine. Leptospira interrogans serovar Copenhageni transmitted from Rattus norvegicus to humans is the most prevalent cause of urban leptospirosis. We examined L. interrogans LigA, domains 7 to 13 (LigA7-13), as an oral vaccine delivered by Escherichia coli as a lipidated, membrane-associated protein. The efficacy of the vaccine was evaluated in a susceptible hamster model in terms of the humoral immune response and survival from leptospiral challenge. Four weeks of oral administration of live E. coli expressing LigA7-13 improved survival from intraperitoneal (i.p.) and intradermal (i.d.) challenge by L. interrogans serovar Copenhageni strain Fiocruz L1-130 in Golden Syrian hamsters. Immunization with E. coli expressing LigA7-13 resulted in a systemic antibody response, and a significant LigA7-13 IgG level after the first 2 weeks of immunization was completely predictive of survival 28 days after challenge. As in previous LigA vaccine studies, all immunized hamsters that survived infection had renal leptospiral colonization and histopathological changes. In summary, an oral LigA-based vaccine improved survival from leptospiral challenge by either the i.p. or i.d. route.
机译：钩端螺旋体病是一种潜在的致命人畜共患病，是由宿主宿主在其肾小管中携带钩端螺旋体并在其尿液中排出细菌而传播的。从褐家鼠传播给人类的问号钩端螺旋体血清型哥本哈根菌是城市钩端螺旋体病最普遍的原因。我们检查了L. interrogans LigA，结构域7至13（LigA7-13），作为由大肠杆菌递送的口服疫苗，是一种脂化的膜相关蛋白。在体液免疫反应和钩端螺旋体攻击的存活率方面，在易感仓鼠模型中评估了疫苗的功效。口服施用表达LigA7-13的大肠杆菌的四周时间提高了金黄色叙利亚仓鼠中问号乳酸杆菌哥本哈根杆菌Fiocruz L1-130菌株腹膜内（i.p.）和皮内（i.d.）攻击的存活率。用表达LigA7-13的大肠杆菌进行免疫会产生全身性抗体应答，并且免疫前2周后显着的LigA7-13 IgG水平可以完全预测攻击后28天的存活率。与以前的LigA疫苗研究一样，所有在感染中幸存的免疫仓鼠均具有肾钩端螺旋体定植和组织病理学改变。总之，基于LigA的口服疫苗可通过i.p.免疫接种改善钩端螺旋体攻击的存活率。或路线。

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