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首页> 外文期刊>International Journal of Molecular Sciences >Identification of Differentially Expressed miRNAs between White and Black Hair Follicles by RNA-Sequencing in the Goat (Capra hircus)
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Identification of Differentially Expressed miRNAs between White and Black Hair Follicles by RNA-Sequencing in the Goat (Capra hircus)

机译：通过山羊中的RNA测序鉴定白毛囊和黑毛囊之间差异表达的miRNA。

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摘要

MicroRNAs (miRNAs) play a key role in many biological processes by regulating gene expression at the post-transcriptional level. A number of miRNAs have been identified from livestock species. However, compared with other animals, such as pigs and cows, the number of miRNAs identified in goats is quite low, particularly in hair follicles. In this study, to investigate the functional roles of miRNAs in goat hair follicles of goats with different coat colors, we sequenced miRNAs from two hair follicles samples (white and black) using Solexa sequencing. A total of 35,604,016 reads were obtained, which included 30,878,637 clean reads (86.73%). MiRDeep2 software identified 214 miRNAs. Among them, 205 were conserved among species and nine were novel miRNAs. Furthermore, DESeq software identified six differentially expressed miRNAs. Quantitative PCR confirmed differential expression of two miRNAs, miR-10b and miR-211. KEGG pathways were analyzed using the DAVID website for the predicted target genes of the differentially expressed miRNAs. Several signaling pathways including Notch and MAPK pathways may affect the process of coat color formation. Our study showed that the identified miRNAs might play an essential role in black and white follicle formation in goats.
机译：MicroRNA（miRNA）通过在转录后水平调控基因表达在许多生物学过程中发挥关键作用。从牲畜物种中已经鉴定出许多miRNA。但是，与其他动物（如猪和牛）相比，山羊中鉴定出的miRNA数量非常低，尤其是在毛囊中。在这项研究中，为了研究miRNA在不同皮毛颜色的山羊的山羊毛囊中的功能作用，我们使用Solexa测序对来自两个毛囊样品（白色和黑色）的miRNA进行了测序。总共获得了35,604,016个读段，其中包括30,878,637个干净读段（86.73％）。 MiRDeep2软件识别了214个miRNA。其中，有205个物种保守，有9个是新颖的miRNA。此外，DESeq软件识别了六个差异表达的miRNA。定量PCR证实了两种miRNA miR-10b和miR-211的差异表达。使用DAVID网站分析KEGG途径，以预测差异表达miRNA的预测靶基因。包括Notch和MAPK通路在内的几种信号通路可能会影响外套颜色形成的过程。我们的研究表明，鉴定出的miRNA在山羊黑色和白色卵泡形成中可能起重要作用。

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