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首页> 外文期刊>International Journal of Molecular Sciences >Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus
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Multiplex Hydrolysis Probe Real-Time PCR for Simultaneous Detection of Hepatitis A Virus and Hepatitis E Virus

机译:多重水解探针实时荧光定量PCR同时检测甲型肝炎病毒和戊型肝炎病毒

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Detection of hepatitis viral infections has traditionally relied on the circulating antibody test using the enzyme-linked immunosorbent assay. However, multiplex real-time PCR has been increasingly used for a variety of viral nucleic acid detections and has proven to be superior to traditional methods. Hepatitis A virus (HAV) and hepatitis E virus (HEV) are the major causes of acute hepatitis worldwide; both HAV and HEV infection are a main public health problem. In the present study, a one-step multiplex reverse transcriptase quantitative polymerase chain reaction assay using hydrolysis probes was developed for simultaneously detecting HAV and HEV. This novel detection system proved specific to the target viruses, to be highly sensitive and to be applicable to clinical sera samples, making it useful for rapid, accurate and feasible identification of HAV and HEV.
机译:肝炎病毒感染的检测传统上依靠使用酶联免疫吸附测定的循环抗体测试。但是,多重实时PCR已越来越多地用于各种病毒核酸检测,并已证明优于传统方法。甲型肝炎病毒(HAV)和戊型肝炎病毒(HEV)是全世界急性肝炎的主要原因。 HAV和HEV感染都是主要的公共卫生问题。在本研究中,开发了一种使用水解探针的一步多路逆转录酶定量聚合酶链反应测定法,用于同时检测HAV和HEV。这种新颖的检测系统被证明对目标病毒具有特异性,具有高度的敏感性,并适用于临床血清样品,因此可用于快速,准确和可行的HAV和HEV鉴定。

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