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Francisella tularensis, Portugal

机译：葡萄牙弗朗西斯菌

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To the Editor: Tularemia is azoonosis caused by Francisellatularensis. Recently, tularemia hasemerged in new locations, popula-tions, and settings (1). After an out-break in Spain in 1997 (2), it wasexpected that the disease wouldspread toward Portugal, a countrywith an extended area that borders theaffected areas.To evaluate the situation, a sur-veillance project, including a seroepi-demiologic study in human popula-tions and detection of the nucleic acidof F. tularensis in biologic samples,was initiated. The district ofBragan.a, in northern Portugal, wasselected as study area for its vicinitywith tularemia-endemic areas ofSpain and because Dermacentor retic-ulatus and Ixodes ricinus are welldocumented there (3).Biologic samples were collectedfrom 74 persons living in the studyregion whose activities represented anincreased risk for contact with ticksand wild mammals. Serum sampleswere available from 48 and were ana-lyzed with the microagglutination test(4). From the other 26 persons, bloodsamples were collected and frozen.Because of hemolization these sam-ples were only subjected to PCR.DNA was extracted by using theQIAamp blood kit (QIAGEN GmbH,Hilden, Germany).
机译：致编者：Tularemia是由Francisellatularensis引起的无语症。最近，tularemia出现在新的位置，人口和环境中（1）。在1997年西班牙爆发疫情之后（2），预计该病会蔓延到葡萄牙，该国的受灾地区与受灾地区接壤。为了评估这种状况，开展了一项监测项目，其中包括西班牙的血清流行病学研究。启动了人类种群并检测了生物样品中的土拉弗朗西斯菌核酸。葡萄牙北部布拉干区被选为研究区，因为它与西班牙的图莱血病流行区相邻，并且因为那里的文献充分记载了Dermacentor retic-ulatus和Ixodes ricinus（3）。从该研究区域生活的74人中收集了生物学样本代表与tick和野生哺乳动物接触的风险增加。血清样本来自48个，并通过微凝集试验进行了分析（4）。从其他26个人中收集血液样本并冷冻。由于血液化，仅对这些样本进行PCR。使用QIAamp血液试剂盒（QIAGEN GmbH，Hilden，德国）提取DNA。

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