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首页> 外文期刊>Molecular and Cellular Biology >Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.
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Complex protein binding within the mouse immunoglobulin heavy-chain enhancer.

机译：小鼠免疫球蛋白重链增强剂中的复杂蛋白结合。

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摘要

We have begun to purify and characterize several proteins which bind to the mouse immunoglobulin heavy-chain enhancer to understand the molecular interactions important for enhancer activity. Three proteins which bind to different sites on the immunoglobulin heavy-chain enhancer have been chromatographically separated and partially purified. One protein binds a site which has not been reported previously and does not bind to other reported protein-binding sites on the immunoglobulin heavy-chain enhancer. Binding-site boundaries for the three partially purified proteins have been precisely mapped by methylation interference, DNase I footprinting, and orthophenanthroline/copper chemical nuclease footprinting. We have also characterized these three proteins with respect to dissociation rate constants.
机译：我们已经开始纯化和表征与小鼠免疫球蛋白重链增强子结合的几种蛋白质，以了解对于增强子活性重要的分子相互作用。色谱分离并部分纯化了与免疫球蛋白重链增强子上不同位点结合的三种蛋白质。一种蛋白质结合先前未报道的位点，并且不结合免疫球蛋白重链增强子上其他报道的蛋白质结合位点。三种部分纯化的蛋白质的结合位点边界已通过甲基化干扰，DNase I足迹和邻菲咯啉/铜化学核酸酶足迹精确定位。我们还针对解离速率常数表征了这三种蛋白质。

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