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首页> 外文期刊>Molecular and Cellular Biology >Expression of mouse histone genes: transcription into 3' intergenic DNA and cryptic processing sites downstream from the 3' end of the H3 gene.
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Expression of mouse histone genes: transcription into 3' intergenic DNA and cryptic processing sites downstream from the 3' end of the H3 gene.

机译：小鼠组蛋白基因的表达：转录到3'基因间DNA和H3基因3'末端下游的密码加工位点。

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Introduction of the mouse histone H3.1 gene into tk- mouse L cells by cotransfection with the herpesvirus thymidine kinase gene resulted in the production of two mRNAs from the transfected gene, one with a normal 3' end and the other one with a longer 3'-untranslated region, ending at site X, which was poly(A)+. In contrast, the endogenous histone H3.1 gene only produced a single mRNA. The cryptic poly(A)+ site was only used when the histone H3.1 gene was transfected. To localize possible downstream cryptic processing sites, the hairpin loop at the end of the histone gene was deleted and the resulting deletions were introduced into L cells. Two major mRNAs were produced from this gene, one ending at site X and the major one ending at site Y, which was located 150 nucleotides before site X. Transcription extended downstream of site X efficiently in the endogenous gene, as judged by the extent of transcription of downstream sequences in isolated nuclei. Transcription extended downstream of site X in the transfected gene because the placement of a normal histone 3' end downstream of site X resulted in transcripts that ended at site X and longer transcripts that ended with the new histone 3' end. These results indicate that transcription may normally proceed a substantial distance past the hairpin loop (greater than 500 bases). The formation of the different 3' ends in these transfected genes was due to competition between different processing mechanisms.
机译：通过与疱疹病毒胸苷激酶基因共转染将小鼠组蛋白H3.1基因导入tk-小鼠L细胞，导致转染的基因产生了两个mRNA，一个具有3'端正常，另一个具有更长的3'mRNA。 '-非翻译区，终止于位点X，为poly（A）+。相反，内源性组蛋白H3.1基因仅产生单个mRNA。仅在转染组蛋白H3.1基因时才使用隐蔽的poly（A）+位点。为了定位可能的下游密码子加工位点，删除了组蛋白基因末端的发夹环，并将产生的缺失引入L细胞。从该基因产生了两个主要的mRNA，一个主要终止于X位，另一个主要终止于Y位，其位于X位点之前的150个核苷酸处。根据内源基因的程度判断，转录有效地延伸到X位的下游。分离核中下游序列的转录。转录延伸至转染基因中位点X的下游，因为将正常组蛋白3'末端置于位点X的下游，导致转录物在位点X终止，而更长的转录物以新的组蛋白3'末端终止。这些结果表明，转录通常可能会经过发夹环一段相当长的距离（大于500个碱基）。这些转染的基因中不同3'末端的形成是由于不同加工机制之间的竞争。

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