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首页> 外文期刊>Molecular and Cellular Biology >An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.
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An intact histone 3'-processing site is required for transcription termination in a mouse histone H2a gene.

机译：小鼠组蛋白H2a基因的转录终止需要完整的组蛋白3'-加工位点。

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A transcription termination site has been characterized between the mouse histone H2a-614 and H3-614 genes. There is a poly(A)- RNA present in small amounts in the nucleus which ends 600 nucleotides 3' to the H2a-614 gene. Nuclear transcription studies demonstrate that transcription extends at least 600 nucleotides 3' to the gene but is greatly reduced 700 nucleotides 3' to the gene. If all or part of the normal 3'-processing signal, consisting of the stem-loop and the U7 small nuclear ribonucleoprotein binding site, is deleted, transcription then continues past the putative termination site and RNAs which end at the 3' end of the downstream H3-614 gene accumulate. Insertion of a 150-nucleotide fragment containing the termination site between the histone 3' end and downstream polyadenylation sites reduces usage of polyadenylation sites 85 to 90%. Taken together these results suggest there is a transcription termination site which requires an intact histone 3'-processing signal to function.
机译：已经在小鼠组蛋白H2a-614和H3-614基因之间表征了转录终止位点。在细胞核中存在少量的poly（A）-RNA，该RNA终止于H2a-614基因3'的600个核苷酸。核转录研究表明，转录至少延伸了基因3'的600个核苷酸，但大大减少了基因3'的700个核苷酸。如果由茎环和U7小核糖核糖蛋白结合位点组成的正常3'加工信号的全部或部分被删除，则转录会继续通过假定的终止位点和终止于3'端的RNA。下游H3-614基因积累。在组蛋白3'端和下游聚腺苷酸化位点之间插入包含终止位点的150个核苷酸的片段可将聚腺苷酸化位点的使用降低85％至90％。这些结果加在一起表明存在一个转录终止位点，该位点需要完整的组蛋白3'加工信号起作用。

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