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首页> 外文期刊>Molecular and Cellular Biology >Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.
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Heat shock-induced interactions of heat shock transcription factor and the human hsp70 promoter examined by in vivo footprinting.

机译:通过体内足迹检测热休克诱导的热休克转录因子与人hsp70启动子的相互作用。

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摘要

Genomic footprinting of the human hsp70 promoter reveals that heat shock induces a rapid binding of a factor, presumably heat shock transcription factor, to a region encompassing five contiguous NGAAN sequences, three perfect and two imperfect matches to the consensus sequence. Arrays of inverted NGAAN sequences have been defined as the heat shock element. No protein is bound to the heat shock element prior to or after recovery from heat shock. Heat shock does not perturb the binding of factors to other regulatory elements in the promoter which contribute to basal expression of the hsp70 gene.
机译:人类hsp70启动子的基因组足迹表明,热休克诱导因子(可能是热休克转录因子)与包含五个连续NGAAN序列,三个完全匹配和两个不完全匹配的序列的快速结合。反向NGAAN序列的阵列已被定义为热激元件。在从热激中恢复之前或之后,没有蛋白与热激元件结合。热休克不会干扰启动子中其他因子与hsp70基因基础表达的结合。

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