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首页> 外文期刊>Molecular and Cellular Biology >Tissue-specific expression of human CD4 in transgenic mice.
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Tissue-specific expression of human CD4 in transgenic mice.

机译：人CD4在转基因小鼠中的组织特异性表达。

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摘要

The gene for the human CD4 glycoprotein, which serves as the receptor for human immunodeficiency virus type 1, along with approximately 23 kb of sequence upstream of the translational start site, was cloned. The ability of 5' flanking sequences to direct tissue-specific expression was tested in cell culture and in transgenic mice. A 5' flanking region of 6 kb was able to direct transcription of the CD4 gene in NIH 3T3 cells but did not result in detectable expression in the murine T-cell line EL4 or in four lines of transgenic mice. A larger 5' flanking region of approximately 23 kb directed high-level CD4 transcription in the murine T-cell line EL4 and in three independent lines of transgenic mice. Human CD4 expression in all tissues analyzed was tightly correlated with murine CD4 expression; the highest levels of human CD4 RNA expression were found in the thymus and spleen, with relatively low levels detected in other tissues. Expression of human CD4 protein in peripheral blood mononuclear cells was examined by flow cytometry in these transgenic animals and found to be restricted to the murine CD4+ subset of lymphocytes. Human CD4 protein, detected with an anti-human CD4 monoclonal antibody, was present on the surface of 45 to 50% of the peripheral blood mononuclear cells from all transgenic lines.
机译：克隆了人类CD4糖蛋白的基因，该基因用作人类1型免疫缺陷病毒的受体，以及翻译起始位点上游约23 kb的序列。在细胞培养和转基因小鼠中测试了5'侧翼序列指导组织特异性表达的能力。 6 kb的5'侧翼区域能够指导NIH 3T3细胞中CD4基因的转录，但在鼠T细胞系EL4或四行转基因小鼠中均未检测到表达。大约23 kb的较大5'侧翼区域在鼠T细胞系EL4和三个独立的转基因小鼠系中定向高水平CD4转录。分析的所有组织中人CD4的表达与鼠CD4的表达紧密相关。在胸腺和脾脏中发现最高水平的人类CD4 RNA表达，而在其他组织中检测到相对较低的水平。在这些转基因动物中通过流式细胞术检查了人CD4蛋白在外周血单核细胞中的表达，发现其局限于小鼠淋巴细胞的CD4 +亚群。用抗人CD4单克隆抗体检测到的人CD4蛋白存在于所有转基因品系的外周血单核细胞的45％至50％的表面上。

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