• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Molecular and Cellular Biology >Receptor for bombesin with associated tyrosine kinase activity.
【24h】

Receptor for bombesin with associated tyrosine kinase activity.

机译：蛙皮素的受体具有相关的酪氨酸激酶活性。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The neuropeptide bombesin is known for its potent mitogenic activity on murine 3T3 fibroblasts and other cells. Recently it has been implicated in the pathogenesis of small cell lung carcinoma, in which it acts through an autocrine loop of growth stimulation. Phosphotyrosine (P-Tyr) antibodies have been successfully used to recognize the autophosphorylated receptors for known growth factors. In Swiss 3T3 fibroblasts, phosphotyrosine antibodies identified a 115,000-Mr cell surface protein (p115) that became phosphorylated on tyrosine as a specific response to bombesin stimulation of quiescent cells. The extent of phosphorylation was dose dependent and correlated with the mitogenic effect induced by bombesin, measured by [3H]thymidine incorporation. Tyrosine phosphorylation of p115 was detectable minutes after the addition of bombesin, and its time course paralleled that described for the binding of bombesin to its receptor. Immunocomplexes of phosphorylated p115 and phosphotyrosine antibodies bound 125I-labeled [Tyr4]bombesin in a specific and saturable manner and displayed an associated tyrosine kinase activity enhanced by bombesin. Furthermore, the 125I-labeled bombesin analog gastrin-releasing peptide, bound to intact live cells, was coprecipitated with p115. These data strongly suggest that p115 participates in the structure and function of the surface receptor for bombesin, a new member of the family of growth factor receptors with associated tyrosine kinase activity.
机译：众所周知，神经肽蛙皮素对鼠类3T3成纤维细胞和其他细胞具有强大的促有丝分裂活性。最近，它与小细胞肺癌的发病机理有关，其中它通过生长刺激的自分泌循环起作用。磷酸酪氨酸（P-Tyr）抗体已成功用于识别已知生长因子的自磷酸化受体。在瑞士3T3成纤维细胞中，磷酸酪氨酸抗体鉴定出一种115,000-Mr细胞表面蛋白（p115），该蛋白在酪氨酸上被磷酸化，作为对蛙心素刺激静止细胞的特异性反应。磷酸化的程度是剂量依赖性的，并且与通过[3 H]胸苷掺入法测量的由蛙皮素诱导的促有丝分裂作用相关。在添加蛙皮素后数分钟即可检测到p115的酪氨酸磷酸化，其时间过程与描述的蛙皮素与其受体结合的过程平行。磷酸化的p115和磷酸酪氨酸抗体的免疫复合物以特定且可饱和的方式结合125I标记的[Tyr4] bombesin，并显示了由omblesin增强的相关酪氨酸激酶活性。此外，与完整活细胞结合的125 I标记的蛙蛙素类似物胃泌素释放肽与p115共沉淀。这些数据强烈表明，p115参与了蛙心蛋白表面受体的结构和功能，而蛙心蛋白是具有相关酪氨酸激酶活性的生长因子受体家族的新成员。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号