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首页> 外文期刊>Molecular and Cellular Biology >TFIIA induces conformational changes in TFIID via interactions with the basic repeat.
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TFIIA induces conformational changes in TFIID via interactions with the basic repeat.

机译：TFIIA通过与基本重复序列的相互作用诱导TFIID的构象变化。

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DNA-binding studies with Saccharomyces cerevisiae TFIID point mutants indicated that TFIIA interacts with the basic repeat region of TFIID and induces structural changes. The latter was shown by the ability of TFIIA to compensate for TFIID point mutants defective for DNA binding. Interaction with TFIIA also rendered TFIID binding temperature independent, thus mimicking the effect of removing the nonconserved N terminus of TFIID. In addition, N-terminal truncation of the TFIID point mutants defective for DNA binding mimicked the ability of TFIIA to restore DNA binding of those mutants. Taken together, these results suggest that TFIIA enhances TFIID binding to DNA by eliminating an otherwise inhibitory effect of the nonconserved N terminus of TFIID. Furthermore, analyses of TFIID contact points on DNA and binding studies with TATA-containing oligonucleotide probes showed that TFIIA decreases the effect of sequences flanking the adenovirus major late TATA element on TFIID binding to DNA, suggesting a possible role of TFIIA in allowing TFIID to recognize a wider variety of promoters.
机译：用酿酒酵母TFIID点突变体进行的DNA结合研究表明，TFIIA与TFIDD的基本重复区域相互作用，并诱导结构变化。 TFIIA补偿DNA结合缺陷的TFIID点突变体的能力显示了后者。与TFIIA的相互作用也使TFIID的结合温度独立，从而模仿了去除非保守的TFIID N末端的作用。另外，对DNA结合有缺陷的TFIID点突变体的N末端截短模仿了TFIIA恢复那些突变体的DNA结合的能力。综上，这些结果表明，TFIAA通过消除TFIID的非保守N末端的其他抑制作用来增强TFIID与DNA的结合。此外，对DNA上TFIID接触点的分析和与含TATA的寡核苷酸探针的结合研究表明TFIIA降低了腺病毒主要晚期TATA元件侧翼的序列对TFIID与DNA结合的影响，表明TFIIA在允许TFIID识别方面可能发挥作用各种各样的发起人。

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