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首页> 外文期刊>Molecular and Cellular Biology >An RNA polymerase II transcription factor inactivated in poliovirus-infected cells copurifies with transcription factor TFIID.
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An RNA polymerase II transcription factor inactivated in poliovirus-infected cells copurifies with transcription factor TFIID.

机译：在脊髓灰质炎病毒感染的细胞中失活的RNA聚合酶II转录因子与转录因子TFIID共纯化。

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摘要

Inhibition of host cell RNA polymerase II-mediated transcription by poliovirus infection was studied in vitro. Whole-cell extracts prepared from poliovirus-infected HeLa cells at 3 h postinfection were shown to be deficient in a factor required for specific transcription from the adenovirus major late promoter. Three lines of evidence suggest that transcription factor TFIID is deficient in poliovirus-infected cells. First, the activity required to specifically restore transcription in poliovirus-infected cell extracts was shown to copurify with TFIID through three chromatographic steps. Second, transcription reactions reconstituted with phosphocellulose-derived chromatographic fractions revealed a fourfold decrease in the specific activity of the TFIID-containing fraction prepared from poliovirus-infected cells compared with that of the same fraction prepared from mock-infected cells. Finally, TFIID and the activity required to specifically restore transcription in virus-infected cell extracts were shown to have the same kinetics of heat inactivation. Together, these results suggest that inactivation of TFIID is an early event in the inhibition of host cell RNA polymerase II transcription by poliovirus.
机译：在体外研究了脊髓灰质炎病毒感染对宿主细胞RNA聚合酶II介导的转录的抑制作用。从感染脊髓灰质炎病毒的HeLa细胞在感染后3小时制备的全细胞提取物显示缺乏从腺病毒主要晚期启动子进行特异性转录所需的因子。三行证据表明转录因子TFIID在脊髓灰质炎病毒感染的细胞中缺乏。首先，通过三个色谱步骤，表明在脊髓灰质炎病毒感染的细胞提取物中特异性恢复转录所需的活性与TFIID共纯化。第二，用磷酸纤维素衍生的色谱级分重构的转录反应显示，与从模拟感染细胞制备的相同级分相比，从脊髓灰质炎病毒感染的细胞制备的含TFIID的级分的比活性降低了四倍。最后，显示TFIID和特异性恢复病毒感染的细胞提取物中转录所需的活性具有相同的热灭活动力学。总之，这些结果表明TFIID的失活是脊髓灰质炎病毒抑制宿主细胞RNA聚合酶II转录的早期事件。

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