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首页> 外文期刊>Molecular and Cellular Biology >The Novel ATP-Binding Cassette Protein ARB1 Is a Shuttling Factor That Stimulates 40S and 60S Ribosome Biogenesis
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The Novel ATP-Binding Cassette Protein ARB1 Is a Shuttling Factor That Stimulates 40S and 60S Ribosome Biogenesis

机译：新型的ATP结合盒式蛋白ARB1是刺激40S和60S核糖体生物发生的穿梭因子。

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摘要

ARB1 is an essential yeast protein closely related to members of a subclass of the ATP-binding cassette (ABC) superfamily of proteins that are known to interact with ribosomes and function in protein synthesis or ribosome biogenesis. We show that depletion of ARB1 from Saccharomyces cerevisiae cells leads to a deficit in 18S rRNA and 40S subunits that can be attributed to slower cleavage at the A0, A1, and A2 processing sites in 35S pre-rRNA, delayed processing of 20S rRNA to mature 18S rRNA, and a possible defect in nuclear export of pre-40S subunits. Depletion of ARB1 also delays rRNA processing events in the 60S biogenesis pathway. We further demonstrate that ARB1 shuttles from nucleus to cytoplasm, cosediments with 40S, 60S, and 80S/90S ribosomal species, and is physically associated in vivo with TIF6, LSG1, and other proteins implicated previously in different aspects of 60S or 40S biogenesis. Mutations of conserved ARB1 residues expected to function in ATP hydrolysis were lethal. We propose that ARB1 functions as a mechanochemical ATPase to stimulate multiple steps in the 40S and 60S ribosomal biogenesis pathways.
机译：ARB1是一种必需的酵母蛋白，与已知与核糖体相互作用并在蛋白质合成或核糖体生物发生中起作用的ATP结合盒（ABC）超家族的亚类成员密切相关。我们发现，酿酒酵母细胞中ARB1的耗尽导致18S rRNA和40S亚基的缺乏，这可能归因于35S pre-rRNA中A0，A1和A2加工位点的切割较慢，延缓了20S rRNA加工到成熟的18S rRNA的过程，并可能导致40S之前亚基的核输出缺陷。 ARB1的消耗也会延迟60S生物发生途径中的rRNA加工事件。我们进一步证明，ARB1从细胞核穿梭到细胞质，与40S，60S和80S / 90S核糖体种共沉淀，并且在体内与TIF6，LSG1和先前涉及60S或40S生物发生的不同方面的其他蛋白质发生物理关联。预期在ATP水解中起作用的保守ARB1残基的突变是致命的。我们建议ARB1作为一种机械化学ATPase来刺激40S和60S核糖体生物发生途径中的多个步骤。

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