A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene.

H Zhu; V T Nguyen; A B Brown; A Pourhosseini; A V Garcia; M van Bilsen; K R Chien

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A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene.

机译：一种新型的组织受限锌指蛋白（HF-1b）与大鼠肌球蛋白轻链2基因中的心脏调节元件（HF-1b / MEF-2）结合。

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The AT-rich element MEF-2 plays an important role in the maintenance of the muscle-specific expression of a number of cardiac and skeletal muscle genes. In the MLC-2 gene, an AT-rich element (HF-1b) which contains a consensus MEF-2 site is required for cardiac tissue-specific expression. The present study reports the isolation and characterization of a cDNA which encodes a novel C2H2 zinc finger (HF-1b) that binds in a sequence-specific manner to the HF-1b/MEF-2 site in the MLC-2 promoter. A number of independent criteria suggest that this HF-1b zinc finger protein is a component of the endogenous HF-1b/MEF-2 binding activity in cardiac muscle cells and that it can serve as a transcriptional activator of the MLC-2 promoter in transient assays. These studies suggest that, in addition to the previously reported RSRF proteins, structurally divergent transcriptional factors can bind to MEF-2-like sites in muscle promoters. These results underscore the complexity of the regulation of the muscle gene program via these AT-rich elements in cardiac and skeletal muscle.

机译：富含AT的元素MEF-2在维持许多心脏和骨骼肌基因的肌肉特异性表达中起着重要作用。在MLC-2基因中，心脏组织特异性表达需要含有一个共有MEF-2位点的富含AT的元件（HF-1b）。本研究报告了编码新型C2H2锌指（HF-1b）的cDNA的分离和表征，该C2H2锌指以序列特异性方式与MLC-2启动子中的HF-1b / MEF-2位点结合。许多独立的标准表明，该HF-1b锌指蛋白是心肌细胞中内源性HF-1b / MEF-2结合活性的组成部分，并且可以在瞬时中用作MLC-2启动子的转录激活因子。分析。这些研究表明，除先前报道的RSRF蛋白外，结构上不同的转录因子还可以结合肌肉启动子中的MEF-2样位点。这些结果强调了通过心肌和骨骼肌中这些富含AT的元素来调节肌肉基因程序的复杂性。

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《Molecular and Cellular Biology》 |1993年第7期|共13页
作者
H Zhu; V T Nguyen; A B Brown; A Pourhosseini; A V Garcia; M van Bilsen; K R Chien;
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1. Positive regulatory elements (HF-1a and HF-1b) and a novel negative regulatory element (HF-3) mediate ventricular muscle-specific expression of myosin light-chain 2-luciferase fusion genes in transgenic mice. [J] . K J Lee, R Hickey, H Zhu, Molecular and Cellular Biology . 1994,第2期

机译：正调控元件（HF-1a和HF-1b）和新型负调控元件（HF-3）介导转基因小鼠肌球蛋白轻链2-荧光素酶融合基因的心室肌特异性表达。
2. A ubiquitous factor (HF-1a) and a distinct muscle factor (HF-1b/MEF-2) form an E-box-independent pathway for cardiac muscle gene expression. [J] . S Navankasattusas, H Zhu, A V Garcia, Molecular and Cellular Biology . 1992,第4期

机译：普遍存在的因子（HF-1a）和独特的肌肉因子（HF-1b / MEF-2）形成了独立于E-box的心肌基因表达途径。
3. The basic helix-loop-helix protein upstream stimulating factor regulates the cardiac ventricular myosin light-chain 2 gene via independent cis regulatory elements. [J] . S Navankasattusas, M Sawadogo, M van Bilsen, Molecular and Cellular Biology . 1994,第11期

机译：基本的螺旋-环-螺旋蛋白上游刺激因子通过独立的顺式调节元件调节心脏心室肌球蛋白轻链2基因。
4. First-time Identification of Four Protein Kinase A Phosphorylation Sites in Both Murine and Human Isoforms of Cardiac Myosin Binding Protein-C [C] . Weitao Jia, Justin F. Shaffer, Samantha P. Harris, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：四种蛋白激酶的首次鉴定鼠和人同种型的磷酸化位点结合蛋白-c-c
5. Metal-binding properties of the zinc fingers in metal responsive element-binding transcription factor-1 and design of single-stranded nucleic acid binding proteins using nucleocapsid-like CCHC zinc-binding domains. [D] . Guerrerio, Anthony Lawrence, Jr. 2004

机译：锌指在金属反应元件结合转录因子-1中的金属结合特性，以及使用核衣壳样CCHC锌结合结构域设计单链核酸结合蛋白。
6. A novel tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene. [O] . H Zhu, V T Nguyen, A B Brown, 1993

机译：一种新型的组织限制性锌指蛋白（HF-1b）与大鼠肌球蛋白轻链2基因中的心脏调节元件（HF-1b / MEF-2）结合。
7. A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene. [O] . Zhu, H, Nguyen, V T, Brown, A B, 1993

机译：一种新型的组织限制性锌指蛋白（HF-1b）与大鼠肌球蛋白轻链2基因中的心脏调节元件（HF-1b / MEF-2）结合。

A novel, tissue-restricted zinc finger protein (HF-1b) binds to the cardiac regulatory element (HF-1b/MEF-2) in the rat myosin light-chain 2 gene.

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