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首页> 外文期刊>Molecular and Cellular Biology >ASAP1, a Phospholipid-Dependent Arf GTPase-Activating Protein That Associates with and Is Phosphorylated by Src
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ASAP1, a Phospholipid-Dependent Arf GTPase-Activating Protein That Associates with and Is Phosphorylated by Src

机译：ASAP1，一种磷脂依赖性的Arf GTP酶激活蛋白，与Src结合并被其磷酸化

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Membrane trafficking is regulated in part by small GTP-binding proteins of the ADP-ribosylation factor (Arf) family. Arf function depends on the controlled exchange and hydrolysis of GTP. We have purified and cloned two variants of a 130-kDa phosphatidylinositol 4,5-biphosphate (PIP2)-dependent Arf1 GTPase-activating protein (GAP), which we call ASAP1a and ASAP1b. Both contain a pleckstrin homology (PH) domain, a zinc finger similar to that found in another Arf GAP, three ankyrin (ANK) repeats, a proline-rich region with alternative splicing and SH3 binding motifs, eight repeats of the sequence E/DLPPKP, and an SH3 domain. Together, the PH, zinc finger, and ANK repeat regions possess PIP2-dependent GAP activity on Arf1 and Arf5, less activity on Arf6, and no detectable activity on Arl2 in vitro. The cDNA for ASAP1 was independently identified in a screen for proteins that interact with the SH3 domain of the tyrosine kinase Src. ASAP1 associates in vitro with the SH3 domains of Src family members and with the Crk adapter protein. ASAP1 coprecipitates with Src from cell lysates and is phosphorylated on tyrosine residues in cells expressing activated Src. Both coimmunoprecipitation and tyrosine phosphorylation depend on the same proline-rich class II Src SH3 binding site required for in vitro association. By directly interacting with both Arfs and tyrosine kinases involved in regulating cell growth and cytoskeletal organization, ASAP1 could coordinate membrane remodeling events with these processes.
机译：膜运输部分受到ADP-核糖基化因子（Arf）家族的小GTP结合蛋白的调节。 Arf功能取决于GTP的受控交换和水解。我们已经纯化并克隆了一个130kDa磷脂酰肌醇4,5-二磷酸（PIP 2 ）依赖性Arf1 GTP酶激活蛋白（GAP）的两个变体，我们将其称为ASAP1a和ASAP1b。两者都包含一个pleckstrin同源性（PH）域，一个类似于另一个Arf GAP中发现的锌指，三个锚蛋白（ANK）重复序列，一个富含脯氨酸的区域以及其他剪接和SH3结合基序，八个E / DLPPKP重复序列，以及一个SH3域。在体外，PH，锌指和ANK重复区域共同具有对Arf1和Arf5的PIP 2 依赖的GAP活性，对Arf6的活性较小，而对Arl2则没有可检测的活性。在筛选与酪氨酸激酶Src SH3结构域相互作用的蛋白质的过程中，独立鉴定了ASAP1的cDNA。 ASAP1在体外与Src家族成员的SH3结构域和Crk衔接子蛋白相关联。 ASAP1与Src从细胞裂解物中共沉淀，并在表达活化Src的细胞中的酪氨酸残基上被磷酸化。免疫共沉淀和酪氨酸磷酸化均取决于体外缔合所需的相同脯氨酸富集的II类Src SH3结合位点。通过与参与调节细胞生长和细胞骨架组织的Arfs和酪氨酸激酶直接相互作用，ASAP1可以在这些过程中协调膜重塑事件。

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