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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton
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The Arf GTPase-activating protein ASAP1 regulates the actin cytoskeleton

机译：Arf GTPase激活蛋白ASAP1调节肌动蛋白的细胞骨架

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摘要

Arf family GTP-binding proteins are best characterized as regula- tors of membrane traffic, but recent studies indicate an additional role in cytoskeletal organization. An Arf GTPase-activating protein of the centaurin p family, ASAP1 (also known as centaurin β4). hinds Arf and two other known regulators of the actin cytoskel- eton, the tyrosine kinase Src and phosphatidylinositol 4,5-bisphos- Phate. In this paper, we show that ASAP1 localizes to focal adhesions and cycles with focal adhesion proteins when cells are stimulated to move. Overexpression of ASAP1 altered the mor- ghology of focal adhesions and blocked both cell spreading and formation of dorsal ruffles induced by platelet-derived growth factor (PDGF). On the other hand. ASAP1. with a mutation that disrupted GTPase-activating protein activity. had a reduced effect on cell spreading and increased the number of cells forming dorsal ruffles in response to PDGF. These data support a role for an Arf GTPase-activating protein, ASAP1, as a regulator of cytoskeletal remodeling and raise the possibility that the Arf pathway is a target for PDGF signaling.
机译：Arf家族的GTP结合蛋白最能作为膜运输的调节剂，但最近的研究表明在细胞骨架组织中还有其他作用。百夫长蛋白p家族的Arf GTP酶激活蛋白ASAP1（也称为百夫长蛋白β4）。阻碍了Arf和肌动蛋白细胞骨架的两个其他已知调节剂，酪氨酸激酶Src和磷脂酰肌醇4,5-双磷酸酯。在本文中，我们显示当刺激细胞移动时，ASAP1定位于粘着斑和粘着斑蛋白循环。 ASAP1的过表达改变了粘着斑的形态学，并阻断了血小板衍生的生长因子（PDGF）诱导的细胞扩散和背纹的形成。另一方面。尽快1。具有破坏GTPase激活蛋白活性的突变。对PDGF的抑制作用降低了细胞的扩散，并增加了形成背褶的细胞数量。这些数据支持Arf GTP酶激活蛋白ASAP1作为细胞骨架重塑的调节剂，并增加了Arf途径是PDGF信号转导靶标的可能性。

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