A Multiplicity of Coactivators Is Required by Gcn4p at Individual Promoters In Vivo

Mark J. Swanson; Hongfang Qiu; Laarni Sumibcay; Anna Krueger; Soon-ja Kim; Krishnamurthy Natarajan; Sungpil Yoon; Alan G. Hinnebusch

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A Multiplicity of Coactivators Is Required by Gcn4p at Individual Promoters In Vivo

机译：Gcn4p在单个启动子体内需要多种共激活因子

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Transcriptional activators interact with multisubunit coactivators that modify chromatin structure or recruit the general transcriptional machinery to their target genes. Budding yeast cells respond to amino acid starvation by inducing an activator of amino acid biosynthetic genes, Gcn4p. We conducted a comprehensive analysis of viable mutants affecting known coactivator subunits from the Saccharomyces Genome Deletion Project for defects in activation by Gcn4p in vivo. The results confirm previous findings that Gcn4p requires SAGA, SWI/SNF, and SRB mediator (SRB/MED) and identify key nonessential subunits of these complexes required for activation. Among the numerous histone acetyltransferases examined, only that present in SAGA, Gcn5p, was required by Gcn4p. We also uncovered a dependence on CCR4-NOT, RSC, and the Paf1 complex. In vitro binding experiments suggest that the Gcn4p activation domain interacts specifically with CCR4-NOT and RSC in addition to SAGA, SWI/SNF, and SRB/MED. Chromatin immunoprecipitation experiments show that Mbf1p, SAGA, SWI/SNF, SRB/MED, RSC, CCR4-NOT, and the Paf1 complex all are recruited by Gcn4p to one of its target genes (ARG1) in vivo. We observed considerable differences in coactivator requirements among several Gcn4p-dependent promoters; thus, only a subset of the array of coactivators that can be recruited by Gcn4p is required at a given target gene in vivo.

机译：转录激活因子与修饰染色质结构或募集通用转录机制至其靶基因的多亚基共激活因子相互作用。萌芽的酵母细胞通过诱导氨基酸生物合成基因Gcn4p的激活剂来响应氨基酸饥饿。我们对影响 Saccharomyces 基因组删除项目的已知共激活子亚基的可行突变体进行了全面分析，以研究其在体内被Gcn4p激活的缺陷。结果证实了以前的发现，即Gcn4p需要SAGA，SWI / SNF和SRB介体（SRB / MED），并确定激活这些复合物的关键非必需亚基。在检查的众多组蛋白乙酰基转移酶中，Gcn4p仅需要SAGA中存在的组蛋白Gcn5p。我们还发现了对CCR4-NOT，RSC和Paf1复合体的依赖性。体外结合实验表明，除了SAGA，SWI / SNF和SRB / MED外，Gcn4p激活域还与CCR4-NOT和RSC特异性相互作用。染色质免疫沉淀实验表明，Gcn4p在体内将Mbf1p，SAGA，SWI / SNF，SRB / MED，RSC，CCR4-NOT和Paf1复合物募集到其靶基因之一（ ARG1 ）。我们观察到几个Gcn4p依赖的启动子之间的共激活剂需求有相当大的差异。因此，在体内给定的靶基因上，仅需要可被Gcn4p募集的共激活子阵列的一个子集。 展开▼

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《Molecular and Cellular Biology》 |2003年第8期|共21页

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Mark J. Swanson; Hongfang Qiu; Laarni Sumibcay; Anna Krueger; Soon-ja Kim; Krishnamurthy Natarajan; Sungpil Yoon; Alan G. Hinnebusch;
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中图分类细胞生物学;

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专利

1. An Array of Coactivators Is Required for Optimal Recruitment of TATA Binding Protein and RNA Polymerase II by Promoter-Bound Gcn4p [J] . Hongfang Qiu, Cuihua Hu, Sungpil Yoon, Molecular and Cellular Biology . 2004,第10期

机译：通过启动子结合的Gcn4p最佳招募TATA结合蛋白和RNA聚合酶II需要一系列共激活剂。

2. yTAF_(II)61 Has a General Role in RNA Polymerase II Transcription and Is Required by Gcn4p to Recruit the SAGA Coactivator Complex [J] . Krishnamurthy Natarajan, Blinda M. Jackson, Eugene Rhee, Molecular cell . 1998,第5期

机译：yTAF_（II）61在RNA聚合酶II转录中具有一般作用，是Gcn4p招募SAGA共激活物复合物所必需的

3. yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex. [J] . Natarajan K, Jackson BM, Rhee E, Molecular cell . 1998,第5期

机译：yTAFII61在RNA聚合酶II转录中具有一般作用，是Gcn4p募集SAGA共激活复合物所必需的。

4. In Vivo Assessment of Insulin Delivery with a Series of Compounds as Permeability Promoters [C] . Christian M. Choi, Sun Uk Kim, Kyu Sup Shin, Annual meeting exposition of the Controlled Release Society . 2010

机译：用一系列化合物作为渗透促进剂的胰岛素输送的体内评估

5. Myocardin: A powerful SRF-coactivator required for normal smooth muscle and cardiac ventricular development. [D] . Hoofnagle, Mark Houston. 2008

机译：心肌素：一种功能强大的SRF共激活剂，是正常平滑肌和心室发育所必需的。

6. A Multiplicity of Coactivators Is Required by Gcn4p at Individual Promoters In Vivo [O] . Mark J. Swanson, Hongfang Qiu, Laarni Sumibcay, 2003

机译：Gcn4p在单个启动子体内需要多种共激活因子

7. A Multiplicity of Coactivators Is Required by Gcn4p at Individual Promoters In Vivo [O] . Swanson, Mark J., Qiu, Hongfang, Sumibcay, Laarni, 2003

机译：Gcn4p在单个启动子体内需要多种共激活因子

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A Multiplicity of Coactivators Is Required by Gcn4p at Individual Promoters In Vivo

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