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首页> 外文期刊>Molecular cell >yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex.

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yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex.

机译：yTAFII61在RNA聚合酶II转录中具有一般作用，是Gcn4p募集SAGA共激活复合物所必需的。

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We obtained a recessive insertion mutation in the gene encoding yeast TBP-associated factor yTAFII61/68 that impairs Gcn4p-independent and Gcn4p-activated HIS3 transcription. This mutation also reduces transcription of seven other class II genes, thus indicating a broad role for this yTAFII in RNA polymerase II transcription. The Gcn4p activation domain interacts with multiple components of the SAGA complex in cell extracts, including the yTAFII proteins associated with SAGA, but not with two yTAFIIs restricted to TFIID. The taf61-1 mutation impairs binding of Gcn4p to SAGA/yTAFII subunits but not to components of holoenzyme mediator. Our results provide strong evidence that recruitment of SAGA, in addition to holoenzyme, is crucial for activation by Gcn4p in vivo and that yTAFII61 plays a key role in this process.

机译：我们在编码酵母TBP相关因子yTAFII61 / 68的基因中获得了隐性插入突变，该突变削弱了Gcn4p独立和Gcn4p激活的HIS3转录。此突变还减少了其他七个II类基因的转录，因此表明该yTAFII在RNA聚合酶II转录中具有广泛的作用。 Gcn4p激活结构域与细胞提取物中的SAGA复合物的多个成分相互作用，包括与SAGA相关的yTAFII蛋白，但与两个仅限于TFIID的yTAFII不相互作用。 taf61-1突变削弱了Gcn4p与SAGA / yTAFII亚基的结合，但不损害全酶介体的成分。我们的结果提供了有力的证据，表明除全酶外，SAGA的募集对于Gcn4p在体内的激活至关重要，而yTAFII61在此过程中起着关键作用。

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《Molecular cell》 |1998年第5期|共10页
作者
Natarajan K; Jackson BM; Rhee E; Hinnebusch AG;
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正文语种 eng
中图分类分子生物学;细胞生物学;
关键词
Acetyltransferases; DNA-Binding Proteins; Fungal Proteins; Protein Kinases; 乙酰基转移酶类; DNA结合蛋白质类; 真菌蛋白质类; 蛋白激酶类; RNA聚合酶Ⅱ; 酵母菌; 酿酒;

机译：Acetyltransferases;DNA-Binding Proteins;Fungal Proteins;Protein Kinases;乙酰基转移酶类;DNA结合蛋白质类;真菌蛋白质类;蛋白激酶类;RNA聚合酶Ⅱ;酵母菌;酿酒;

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1. yTAFII61 has a general role in RNA polymerase II transcription and is required by Gcn4p to recruit the SAGA coactivator complex. [J] . Natarajan K, Jackson BM, Rhee E, Molecular cell . 1998,第5期

机译：yTAFII61在RNA聚合酶II转录中具有一般作用，是Gcn4p募集SAGA共激活复合物所必需的。
2. An Array of Coactivators Is Required for Optimal Recruitment of TATA Binding Protein and RNA Polymerase II by Promoter-Bound Gcn4p [J] . Hongfang Qiu, Cuihua Hu, Sungpil Yoon, Molecular and Cellular Biology . 2004,第10期

机译：通过启动子结合的Gcn4p最佳招募TATA结合蛋白和RNA聚合酶II需要一系列共激活剂。
3. The SAGA coactivator complex acts on the whole transcribed genome and is required for RNA polymerase II transcription [J] . BonnetJ., WangC.-Y., BaptistaT., Genes and Development: a Journal Devoted to the Molecular Analysis of Gene Expression in Eukaryotes, Prokaryotes, and Viruses . 2014,第18期

机译：SAGA共激活物复合物作用于整个转录的基因组，是RNA聚合酶II转录所必需的
4. Activation by c-Myc of transcription by RNA polymerases I,II and III [C] . Natividad Gomez-Roman, Zoe A.Felton-Edkins, Niall S.Kenneth Biochemical Society Symposium . 2006

机译：通过RNA聚合酶I，II和III的转录C-MYC激活
5. Molecular studies of RNA polymerase II transcription: Regulation by the NF-kappa B/I kappa B system and identification, purification, cloning, and characterization of coactivators. [D] . Kretzschmar, Marcus. 1995

机译：RNA聚合酶II转录的分子研究：NF-κB/ IκB系统的调控以及共激活因子的鉴定，纯化，克隆和表征。
6. An Array of Coactivators Is Required for Optimal Recruitment of TATA Binding Protein and RNA Polymerase II by Promoter-Bound Gcn4p [O] . Hongfang Qiu, Cuihua Hu, Sungpil Yoon, 2004

机译：通过启动子结合的Gcn4p最佳招募TATA结合蛋白和RNA聚合酶II需要一系列共激活剂。
7. An Array of Coactivators Is Required for Optimal Recruitment of TATA Binding Protein and RNA Polymerase II by Promoter-Bound Gcn4p [O] . Qiu, Hongfang, Hu, Cuihua, Yoon, Sungpil, 2004

机译：通过启动子结合的Gcn4p最佳招募TATA结合蛋白和RNA聚合酶II需要一系列共激活剂。

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