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首页> 外文期刊>Molecular and Cellular Biology >The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein.
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The p55 subunit of Drosophila chromatin assembly factor 1 is homologous to a histone deacetylase-associated protein.

机译：果蝇染色质组装因子1的p55亚基与组蛋白脱乙酰基酶相关蛋白同源。

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摘要

To gain a better understanding of DNA replication-coupled chromatin assembly, we have isolated the cDNA encoding the smallest (apparent molecular mass, 55 kDa; termed p55) subunit of Drosophila melanogaster chromatin assembly factor 1 (dCAF-1), a multisubunit protein that is required for the assembly of nucleosomes onto newly replicated DNA in vitro. The p55 polypeptide comprises seven WD repeat motifs and is homologous to the mammalian RbAp48 protein, which is associated with the HD1 histone deacetylase. dCAF-1 was immunopurified by using affinity-purified antibodies against p55; the resulting dCAF-1 preparation possessed the four putative subunits of dCAF-1 (p180, p105, p75, and p55) and was active for DNA replication-coupled chromatin assembly. Moreover, dCAF-1 activity was specifically depleted with antibodies against p55. Thus, p55 is an integral component of dCAF-1. p55 is localized to the nucleus and is present throughout Drosophila development. Consistent with the homology between p55 and the HD1-associated RbAp48 protein, histone deacetylase activity was observed to coimmunoprecipitate specifically with p55 from a Drosophila nuclear extract. Furthermore, a fraction of the p55 protein becomes associated with the newly assembled chromatin following DNA replication. These findings collectively suggest that p55 may function as a link between DNA replication-coupled chromatin assembly and histone modification.
机译：为了更好地理解与DNA复制相关的染色质组装，我们分离了编码果蝇黑腹果蝇染色质组装因子1（dCAF-1）最小的（表观分子量，55 kDa;称为p55）亚基的cDNA，在体外将核小体装配到新复制的DNA上是必需的。 p55多肽包含七个WD重复基序，与哺乳动物RbAp48蛋白同源，该蛋白与HD1组蛋白脱乙酰基酶相关。使用针对p55的亲和纯化抗体对dCAF-1进行免疫纯化;所得的dCAF-1制剂具有dCAF-1的四个假定的亚基（p180，p105，p75和p55），并且对DNA复制偶联的染色质组装具有活性。此外，dCAF-1活性被针对p55的抗体特异性地耗尽。因此，p55是dCAF-1的组成部分。 p55位于细胞核，在果蝇的整个发育过程中都存在。与p55和与HD1相关的RbAp48蛋白之间的同源性一致，观察到组蛋白脱乙酰基酶活性与果蝇核提取物中的p55特异性共免疫沉淀。此外，DNA复制后，一部分p55蛋白与新组装的染色质相关。这些发现共同表明，p55可能充当DNA复制偶联染色质组装与组蛋白修饰之间的联系。

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