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首页> 外文期刊>Molecular and Cellular Biology >Studies of the Role of the Drosophila scs and scs′ Insulators in Defining Boundaries of a Chromosome Puff
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Studies of the Role of the Drosophila scs and scs′ Insulators in Defining Boundaries of a Chromosome Puff

机译：果蝇scs和scs的绝缘子在定义粉扑边界中的作用研究

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摘要

Insulators are DNA elements that establish independent transcriptional domains within eukaryotic genomes. The Drosophila scs and scs′ insulators localize near the borders of a structural domain in the polytene chromosomes, known as a puff, produced by transcription of the 87A heat shock protein (hsp) genes. It has been suggested that scs and scs′ are boundary elements that delimit this decondensed chromatin domain, reflecting the mechanism by which these sequences act to constrain regulatory interactions. This model was tested using transposons that carried a yellow gene to assess enhancer blocking and an hsp70-lacZ gene to examine the structure of a heat shock puff in the presence and absence of insulators. We found that although scs and scs′ blocked enhancer function, these sequences did not prevent the spread of decondensation resulting from hsp70-lacZ transcription. Further analysis of the endogenous 87A locus demonstrated that scs and scs′ reside within, not at, the borders of the puff. Taken together, our studies suggest that scs and scs′ are not boundary elements that block the propagation of an altered chromatin state associated with puff formation. We propose that these insulators may have a direct role in limiting regulatory interactions in the gene-dense 87A region.
机译：绝缘子是在真核基因组内建立独立转录域的DNA元件。 果蝇 scs和scs的绝缘子位于 87A热激蛋白（ em> hsp ）基因。已经提出，scs和scs'是界定该缩合的染色质结构域的边界元件，反映了这些序列通过其作用来限制调节相互作用的机制。使用带有 yellow 基因以评估增强子阻断作用的转座子和 hsp70-lacZ 基因以检测是否存在热休克粉扑的结构的转座子对该模型进行了测试。绝缘子。我们发现，尽管scs和scs'阻断了增强子的功能，但这些序列并不能阻止因 hsp70-lacZ 转录而引起的缩聚反应的扩散。对内源性 87A 基因座的进一步分析表明，scs和scs'位于粉扑边界内，而不是位于粉扑边界内。综上所述，我们的研究表明，scs和scs'不是阻碍与粉扑形成有关的染色质状态改变传播的边界元素。我们建议这些绝缘子可能在限制基因密集的 87A 区域的调控相互作用中具有直接作用。

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