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首页> 外文期刊>RSC Advances >Study on alterations of physiological functions in aged constipation rats with fluid-deficiency based on metabonomic and microbiology analysis
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Study on alterations of physiological functions in aged constipation rats with fluid-deficiency based on metabonomic and microbiology analysis

机译：基于代谢组学和微生物学分析的老年体液缺乏型便秘大鼠生理功能改变的研究

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Constipation, a common and complex clinical symptom, is a predisposing factor for many serious diseases, such as gastrointestinal diseases, cardiovascular diseases and cancers. Some reports suggest that constipation is associated with gut microbiota, but the specific mechanism is unknown. To clarify how constipation interferes with the normal physiological function of organisms, 1H NMR profiles combined with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16s rRNA gene sequencing were used to investigate the relationship among constipation, metabolism of gut microbiota and host. 27 urinary metabolites and 22 faecal metabolites were found to be associated with constipation, which affect the metabolism of energy, butyric acid, choline, amino acid and the functions of liver and kidney. 5 biomarkers were screened to diagnose the constipation based on the receiver operating characteristic (ROC) analysis. Meanwhile, the levels of Lactobacillus, Intestinimonas, Muribaculum, Eubacterium were significantly decreased, whereas levels of Actinomyces, Culturomica, Ruminococcus, Prevotella, Ruminiclostridium and Acinetobacter were significantly increased. Moreover, the above altered microbiota showed a strong correlation with the metabolites of short chain fatty acids, amino acids, bile acids, choline, and the intermediate product of tricarboxylic acid cycle. These results implied that constipation could result in intestinal dysbacteriosis and metabolism disorder, thereby affecting some normal physiological functions in host. The relationship between gut microbiota and metabolites revealed the potential hazard of constipation from the perspectives of metabonomic and microbiology, and provided new avenues for the diagnosis of constipation and potential drug targets in preventing related diseases.
机译：便秘是一种常见且复杂的临床症状，是许多严重疾病（例如胃肠道疾病，心血管疾病和癌症）的诱发因素。一些报告表明，便秘与肠道菌群有关，但具体机制尚不清楚。为阐明便秘如何干扰生物体的正常生理功能， 1 H NMR谱结合聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）和16s rRNA基因测序用于研究便秘，肠道菌群代谢与宿主之间的关系。便秘与27种尿代谢产物和22种粪便代谢产物有关，它们影响能量，丁酸，胆碱，氨基酸和肝肾功能的代谢。根据接收器工作特征（ROC）分析，筛选出5种生物标志物以诊断便秘。同时，乳酸杆菌，肠小肠，霉菌，真细菌的水平明显降低，而放线菌， Culturomica ， Ruminococcus ， Prevotella ， Ruminiclostridium 和不动杆菌明显增加。此外，上述改变的微生物群显示出与短链脂肪酸，氨基酸，胆汁酸，胆碱和三羧酸循环的中间产物的代谢产物强烈相关。这些结果暗示便秘可能导致肠道菌群失调和代谢紊乱，从而影响宿主的某些正常生理功能。肠道菌群与代谢产物之间的关系从代谢组学和微生物学的角度揭示了便秘的潜在危险，并为便秘的诊断和预防相关疾病的潜在药物靶标提供了新途径。

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