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A sensitive fluorescent probe for alkaline phosphatase and an activity assay based on the aggregation-induced emission effect

机译：碱性磷酸酶敏感的荧光探针和基于聚集诱导发射效应的活性测定

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摘要

In this work, we report a fluorescent probe (TPEQN-P) for detecting alkaline phosphatase (ALP) with high sensitivity and monitoring its activity based on the specific aggregation-induced emission (AIE) effect. TPEQN-P can be constructed by conjugating the phosphate moiety into an AIE molecule (TPE-QI) through a reactive p -hydroxybenzyl group, which exhibits very weak emission in aqueous media due to its good water solubility. In the presence of ALP, TPEQN-P undergoes dephosphorylation and releases the hydrolysis product TPE-QI, which is intensely fluorescent because of its poor water solubility. The detection limit for ALP using TPEQN-P can be as low as 0.0077 U L ~(?1) with a linear range of 0–30 mU mL ~(?1) in solution. TPEQN-P also shows excellent applicability in serum samples, demonstrating potential applications in clinical diagnosis and biomedical research. TPEQN-P also can be applied for the determination of ALP activity and in ALP inhibitor screening.
机译：在这项工作中，我们报告了一种荧光探针（TPEQN-P），该探针可高灵敏度检测碱性磷酸酶（ALP），并根据特定的聚集诱导发射（AIE）效果监控其活性。 TPEQN-P可以通过反应性对羟基苄基将磷酸盐部分缀合到AIE分子（TPE-QI）中来构建，该对羟基苄基由于其良好的水溶性而在水性介质中的发射非常弱。在ALP存在下，TPEQN-P进行去磷酸化并释放出水解产物TPE-QI，由于其水溶性差，该产物强烈发出荧光。使用TPEQN-P对ALP的检出限可低至0.0077 U L〜（？1），溶液中线性范围为0–30 mU mL〜（？1）。 TPEQN-P在血清样品中也显示出优异的适用性，证明了其在临床诊断和生物医学研究中的潜在应用。 TPEQN-P也可用于ALP活性的测定和ALP抑制剂的筛选。

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