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首页> 外文期刊>RSC Advances >A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of Microthrix parvicella
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A novel fluorescent long-chain fatty acid-substituted dye: labeling and biodegrading of Microthrix parvicella

机译：一种新型的荧光长链脂肪酸取代染料：Microthrix parvicella的标记和生物降解

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Microthrix parvicella ( M. parvicella ) is a filamentous bacterium that induces bulking in activated sludge. Here, we used the affinity of long-chain fatty acids (LCFA) for M. parvicella to create a novel fluorescent probe of carbazole modified by LCFA. The structure was characterized by ~(1) H NMR spectroscopy and mass spectrometry. The spectral properties, photostability, and hydrophobic properties of the probe were also characterized. Fluorescent-labeling results showed that it can label M. parvicella in situ and could be biodegraded via metabolism. The stable docking mode of carbazole probes with different fatty acid chains and lipases was also docked by the density functional tight-binding (DFTB) method.
机译：Microthrix parvicella（M. parvicella）是一种丝状细菌，会引起活性污泥中的膨胀。在这里，我们使用长链脂肪酸（LCFA）对细小分支杆菌的亲和力来创建由LCFA修饰的咔唑的新型荧光探针。该结构通过〜（1）1 H NMR光谱和质谱表征。还对探针的光谱性质，光稳定性和疏水性质进行了表征。荧光标记结果表明，它可以原位标记细小支原体，并可以通过代谢被生物降解。还通过密度泛函紧密结合（DFTB）方法对具有不同脂肪酸链和脂肪酶的咔唑探针的稳定对接模式。

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