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首页> 外文期刊>Journal of Clinical Microbiology >Comparison of Legionella pneumophila, L. micdadei, L. bozemanii, and L. dumoffii by transmission electron microscopy.
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Comparison of Legionella pneumophila, L. micdadei, L. bozemanii, and L. dumoffii by transmission electron microscopy.

机译:通过透射电子显微镜比较嗜肺军团菌,米氏乳杆菌,波兹曼乳杆菌和杜氏乳杆菌。

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摘要

Clinical isolates of Legionella pneumophila, L. micdadei, L. bozemanii, and L. dumoffii were grown on charcoal-yeast extract agar from a living-medium inoculum and prepared for transmission electron microscopy by three different methods. Cells of all four Legionella species possessed cytoplasmic vacuoles, a gram-negative type of cell envelope with a dense peptidoglycan-like layer, a ruthenium red-positive polysaccharide capsule, and a single subpolar flagellum. The dense polysaccharide capsule seen on cells of L. micdadei was separated from the outer membrane by an extra layer of electron-lucent material that was not present on cells of the other species examined.
机译:将军团杆菌,米氏乳杆菌,波兹曼乳杆菌和杜氏乳杆菌的临床分离株生长在活体接种物的木炭-酵母提取琼脂上,并通过三种不同的方法制备用于透射电镜。所有四个军团菌物种的细胞都具有胞质液泡,革兰氏阴性类型的细胞膜和稠密的肽聚糖样层,钌红阳性多糖囊和单个亚极鞭毛。在密克氏乳杆菌细胞上见到的致密多糖胶囊通过外层电子透明材料与外膜分离,该层不存在于其他受检种的细胞上。

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