...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Development and evaluation of a biochemical scheme for identification of endocervical lactobacilli.
【24h】

Development and evaluation of a biochemical scheme for identification of endocervical lactobacilli.

机译：鉴定宫颈内生乳杆菌的生化方案的开发和评估。

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A biochemical scheme for the species identification of endocervical lactobacilli was developed and evaluated with 10 isolates obtained from the American Type Culture Collection (ATCC) and 106 endocervical isolates obtained from women reporting to a local venereal disease clinic and a local hospital clinic. The scheme consisted of two stages. Stage I included six tests and was tested and modified with results obtained with ATCC strains. From the modified stage I, stage II was developed. Tests to be performed in this stage were determined from expected characteristics of lactobacilli. Stage II was also tested with the ATCC strains. Of the 106 endocervical isolates, 78 (74%) were identified with the two-stage scheme as developed with the ATCC strains. Unexpected results were obtained in one or both stages with the other 28 isolates. For 10 isolates, the final species identified were not previously expected to be recovered. A "best-fit" method was used to determine the most likely identification of the remaining 18 isolates. In a few instances, the use of a third stage was necessary to reach an identification. The final identification scheme, although complicated in appearance, generated a species identification with a total of 12 tests with a range of 7 to 10 tests per isolate.
机译：制定并评估了一种宫颈内生乳酸菌物种鉴定的生化方案，并从美国典型培养物保藏中心（ATCC）获得了10种分离株，并向当地的性病诊所和当地的医院诊所报告了从女性那里获得的106种宫颈内分离株。该计划包括两个阶段。第一阶段包括六项测试，并通过使用ATCC菌株获得的结果进行了测试和修改。从改进的第一阶段开始，发展第二阶段。根据乳杆菌的预期特征确定在此阶段进行的测试。还用ATCC菌株测试了II期。在106例宫颈分离株中，有78例（74％）通过采用ATCC菌株开发的两阶段方案进行鉴定。在一个或两个阶段中，与其他28个分离株均获得了意外结果。对于10株分离株，最终鉴定出的最终物种以前没有被期望回收。 “最佳拟合”方法用于确定其余18个分离株的最可能鉴定。在少数情况下，必须使用第三阶段才能确定身份。最终的鉴定方案尽管外观复杂，但却产生了一种物种鉴定，共进行了12次检测，每个分离株的检测范围为7至10次。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号