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首页> 外文期刊>Journal of Clinical Microbiology >Molecular analysis of multiple isolates of the major serotypes of group B streptococci.
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Molecular analysis of multiple isolates of the major serotypes of group B streptococci.

机译：B组链球菌主要血清型的多种分离物的分子分析。

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Serotyping of clinical isolates is a widely used technique for epidemiologic study of group B streptococcal infections. However, serotyping cannot definitively determine epidemiologically related or unrelated isolates. We investigated the use of restriction endonuclease analysis (REA) with both conventional agarose gel electrophoresis (AGE) and pulsed-field gel electrophoresis (PFGE) in 50 isolates of the major serotypes of group B streptococci. Single digestion with HindIII and HaeIII and double digestion with HindIII and then EcoRI were used for conventional AGE, and digestion with SmaI was used for PFGE. The molecular profile of one strain was compared with those of the strains within the same serotype as well as with the profiles from strains of different serotypes. Among 10 type Ia, Ia/alpha, Ia/alpha+beta, and Ia/R1 isolates and depending on the restriction enzyme used, we found between five and six REA patterns by conventional AGE and seven by PFGE; among 4 type Ib/alpha+beta isolates we found 2 to 4 REA patterns by conventional AGE and 4 by PFGE; among 21 type II, II/alpha, II/beta, II/alpha+beta, and II/R4 isolates, we found 11 REA patterns by both AGE and PFGE; and among 14 type III, III/R1, and III/R4 isolates, we found from 7 to 12 different REA patterns by AGE and 10 by PFGE. In total, among 13 serotypes and one nontypeable strain, we found 29 to 31 REA patterns by conventional AGE and 33 by PFGE. A particular REA pattern within a serotype was different from the patterns found in the other serotypes, suggesting that REA analysis by using conventional AGE or PFGE is a sensitive method for analyzing genetic relatedness and diversity in group B streptococci and has potential value in molecular epidemiologic studies.
机译：临床分离株的血清分型是B组链球菌感染的流行病学研究中广泛使用的技术。然而，血清分型不能确定流行病学相关或无关的分离株。我们调查了常规琼脂糖凝胶电泳（AGE）和脉冲场凝胶电泳（PFGE）的限制性核酸内切酶分析（REA）在B组链球菌主要血清型的50种分离株中的使用。传统的AGE使用HindIII和HaeIII进行单次消化，然后使用HindIII和EcoRI进行两次消化，而常规GEAGE使用SmaI进行消化。将一种菌株的分子图谱与相同血清型中的菌株的分子图谱以及来自不同血清型的菌株的分子图谱进行比较。在10种Ia，Ia / alpha，Ia / alpha + beta和Ia / R1分离株中，根据所使用的限制酶，我们发现常规AGE中有五到六个REA模式，PFGE中有七个。在4种Ib / alpha + beta分离株中，我们通过常规AGE发现2至4种REA模式，通过PFGE发现4种。在21种II型，II / alpha，II / beta，II / alpha + beta和II / R4分离株中，我们通过AGE和PFGE发现了11种REA模式。在14种III，III / R1和III / R4型分离物中，我们发现AGE有7至12种不同的REA模式，PFGE有10种。总体而言，在13种血清型和1种不可分型的菌株中，我们通过常规AGE发现了29至31种REA模式，通过PFGE发现了33种。血清型中特定的REA模式与其他血清型中的模式不同，这表明使用常规AGE或PFGE进行REA分析是分析B组链球菌遗传相关性和多样性的灵敏方法，在分子流行病学研究中具有潜在价值。

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