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首页> 外文期刊>Journal of Clinical Microbiology >Use of the BacT/Alert Blood Culture System for Culture of Sterile Body Fluids Other than Blood
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Use of the BacT/Alert Blood Culture System for Culture of Sterile Body Fluids Other than Blood

机译：使用BacT / Alert血液培养系统培养血液以外的无菌体液

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Studies have demonstrated that large-volume culture methods for sterile body fluids other than blood increase recovery compared to traditional plated-medium methods. BacT/Alert is a fully automated blood culture system for detecting bacteremia and fungemia. In this study, we compared culture in BacT/Alert standard aerobic and anaerobic bottles, BacT/Alert FAN aerobic and FAN anaerobic bottles, and culture on routine media for six specimen types, i.e., continuous ambulatory peritoneal dialysate (CAPD), peritoneal, amniotic, pericardial, synovial, and pleural fluids. Specimen volumes were divided equally among the three arms of the study. A total of 1,157 specimens were tested, with 227 significant isolates recovered from 193 specimens. Recovery by method was as follows: standard bottles, 186 of 227 (82%); FAN bottles, 217 of 227 (96%); and routine culture, 184 of 227 (81%). The FAN bottles recovered significantly more gram-positive cocci (P < 0.001), Staphylococcus aureus(P = 0.003), coagulase-negative staphylococci (P = 0.008), gram-negative bacilli (P< 0.001), Enterobacteriaceae (P = 0.005), and total organisms (P < 0.001) than the routine culture. There were no significant differences in recovery between the standard bottles and the routine culture. The FAN aerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), coagulase-negative staphyococci (P = 0.003), and total organisms (P< 0.001) than the standard aerobic bottle, while the FAN anaerobic bottle recovered significantly more gram-positive cocci (P < 0.001), S. aureus isolates (P < 0.001), Enterobacteriaceae(P = 0.03), and total organisms (P < 0.001) than the standard anaerobic bottle. For specific specimen types, significantly more isolates were recovered from the FAN bottles compared to the routine culture for synovial (P < 0.001) and CAPD (P = 0.004) fluids. Overall, the FAN bottles were superior in performance to both the standard bottles and the routine culture for detection of microorganisms from the types of sterile body fluids included in this study.
机译：研究表明，与传统的平板培养基方法相比，用于血液以外的无菌体液的大容量培养方法可提高回收率。 BacT / Alert是用于检测菌血症和真菌病的全自动血液培养系统。在这项研究中，我们比较了BacT / Alert标准好氧和厌氧瓶，BacT / Alert FAN好氧和FAN厌氧瓶中的培养，以及常规培养基上六种标本类型的培养，即连续非卧床腹膜透析液（CAPD），腹膜，羊水，心包，滑液和胸膜液。样本量在研究的三个部分之间平均分配。总共测试了1157个样本，从193个样本中回收了227个重要分离株。方法回收如下：标准瓶，227个中的186个（82％）;风扇瓶，227个中的217个（96％）;和常规文化，占227个中的184个（81％）。 FAN瓶的革兰氏阳性球菌（ P <0.001），金黄色葡萄球菌（ P = 0.003），凝固酶阴性葡萄球菌（ P = 0.008），革兰氏阴性杆菌（ P <0.001），肠杆菌科（ P = 0.005），和总生物量（ P <0.001）。标准瓶和常规培养物的回收率没有显着差异。 FAN好氧瓶的革兰氏阳性球菌（ P <0.001）和 S明显多得多。金黄色葡萄球菌（ P <0.001），凝固酶阴性葡萄球菌（ P = 0.003）和总生物体（ P <0.001） ）比标准的需氧瓶更容易，而FAN厌氧瓶的革兰氏阳性球菌的回收率则更高（ P <0.001）， S。金黄色葡萄球菌（ P <0.001），肠杆菌科（emem）（ P = 0.03）和总生物（ P <0.001）比标准厌氧瓶。对于特定的标本类型，与常规培养的滑液（ P <0.001）和CAPD（ P = 0.004）的常规培养相比，从FAN瓶中回收的分离株明显多。总体而言，FAN瓶的性能优于标准瓶和常规培养物，可从本研究中的无菌体液中检测出微生物。

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