Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens

Elizabeth B. Kauffman; Susan A. Jones; Alan P. Dupuis; Kiet A. Ngo; Kristen A. Bernard; Laura D. Kramer

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Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens

机译：西尼罗河病毒在脊椎动物和蚊子标本中的病毒检测协议

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The recent outbreaks of West Nile virus (WNV) infection in the northeastern United States and other regions of the world have made it essential to develop efficient, sensitive, and rapid protocols for virus surveillance. Laboratory testing is the backbone of any surveillance program. Protocols to detect the presence of WNV have been refined since 1999 for sensitivity, speed, efficiency, and specificity. This paper presents the protocols currently used by the New York State Department of Health to handle vertebrate and mosquito specimens that have been submitted for WNV testing to the Arbovirus Laboratories of the Wadsworth Center.

机译：最近在美国东北部和世界其他地区爆发的西尼罗河病毒（WNV）感染使必须开发有效，敏感和快速的病毒监视协议成为必要。实验室测试是任何监视程序的基础。自1999年以来，已针对检测WNV的方法对灵敏度，速度，效率和特异性进行了改进。本文介绍了纽约卫生部目前用于处理脊椎动物和蚊子标本的协议，该标本已提交给沃兹沃思中心的虫媒病毒实验室进行WNV测试。

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《Journal of Clinical Microbiology》 |2003年第8期|共7页
作者
Elizabeth B. Kauffman; Susan A. Jones; Alan P. Dupuis; Kiet A. Ngo; Kristen A. Bernard; Laura D. Kramer;
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中图分类医学微生物学（病原细菌学、病原微生物学）;
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1. A SIMPLE MODIFICATION TO THE MOSQUITO HOMOGENIZATION PROTOCOL SAFELY INACTIVATES WEST NILE VIRUS AND ALLOWS VIRUS DETECTION BY THE RAPID ANALYTE MEASUREMENT PLATFORM (RAMP (R)) ASSAY [J] . Burkhalter Kristen L., Biggerstaff Brad J., Horiuchi Kalanthe, Journal of the American Mosquito Control Association. . 2016,第2期

机译：蚊子均质化协议的简单修改就可以快速激活西尼罗河病毒，并通过快速的分析物测量平台（RAMP（R））检测来检测病毒
2. Serosurvey of West Nile virus in horses and detection of West Nile virus antigen in mosquitoes in Kaduna State, Nigeria [J] . Everest O. ATADIOSE, Junaidu KABIR, Shuaibu G. ADAMU, Journal of Equine Science . 2020,第3期

机译：西尼罗河在尼日利亚岛蚊子的马匹和西尼罗河病毒抗原的马匹和检测的血清
3. Substitution of the premembrane and envelope protein genes of Modoc virus with the homologous sequences of West Nile virus generates a chimeric virus that replicates in vertebrate but not mosquito cells [J] . Rungrat Saiyasombat, Jimena Carrillo-Tripp, Wyatt A Miller, Virology Journal . 2014,第1期

机译：用西尼罗河病毒的同源序列取代Modoc病毒的前膜和包膜蛋白基因，产生一种嵌合病毒，该病毒在脊椎动物而非蚊子细胞中复制
4. Evaluation of the Efficacy Provided by a Recombinant Canarypox-Vectored Equine West Nile Virus Vaccine Against an Experimental West Nile Virus Intrathecal Challenge in Horses [C] . Leonardo Siger, Richard A. Bowen, Kemal Karaca, Annual Convention of the American Association of Equine Practitioners . 2007

机译：评估重组Canarypox的疗效，将马西尼亚尼罗河病毒疫苗对马匹的实验西尼罗河病毒鞘内挑战进行评估
5. The Seroprevalence of West Nile Virus in Human and Mosquito Populations Across the United States of America [D] . Wiercinski, Emily . 2020

机译：在美利坚合众国的人类和蚊虫人口中西尼罗河病毒的Seroprevalence
6. Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens [O] . Elizabeth B. Kauffman, Susan A. Jones, Alan P. Dupuis II, 2003

机译：西尼罗河病毒在脊椎动物和蚊子标本中的病毒检测协议
7. Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens [O] . Kauffman, Elizabeth B., Jones, Susan A., Dupuis II, Alan P., 2003

机译：西尼罗河病毒在脊椎动物和蚊子标本中的病毒检测协议

Virus Detection Protocols for West Nile Virus in Vertebrate and Mosquito Specimens

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