...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Clinical Microbiology >Use of an Anti-Hepatitis C Virus (HCV) IgG Avidity Assay To Identify Recent HCV Infection
【24h】

Use of an Anti-Hepatitis C Virus (HCV) IgG Avidity Assay To Identify Recent HCV Infection

机译：抗丙型肝炎病毒（HCV）IgG亲和力测定法用于鉴定近期HCV感染的用途

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There is no reliable and simple diagnostic marker available to diagnose recent hepatitis C virus (HCV) infection. It has been shown that the avidity of specific IgG antibody is low in primary viral infection and increases with time. We report the development of an anti-HCV avidity assay derived from a commercially available test. A panel of 117 sera was first examined for IgG avidity. It was composed of samples from patients with recent (group 1, n = 14), chronic (group 2, n = 70), and resolved (group 3, n = 33) HCV infections. Avidity index (AI) values observed in recently infected patients were significantly lower (12.0% ± 9.2% [mean ± standard deviation]) than those found in chronic carriers (83.1% ± 15.2%). Using a threshold of 43.0%, this assay distinguished between groups 1 and 2 with very high sensitivity (98%) and specificity (100%). For group 3, a broader distribution of the AI values was observed (54.8% ± 27.3%), suggesting that this index would not be useful in HCV RNA-negative patients. Blind validation of the test was carried out with a panel of 36 serum samples from 17 HCV seroconverters. The assay described here is a useful tool to distinguish recent from chronic infection in HCV-viremic patients.
机译：没有可靠且简单的诊断标记可用于诊断最近的丙型肝炎病毒（HCV）感染。已经显示，在初次病毒感染中特异性IgG抗体的亲和力低，并且随时间增加。我们报告了抗丙肝抗体亲和力测定法的发展，该测定法来自可商购的测试。首先检查一组117个血清的IgG亲和力。它由近期（第1组， n = 14），慢性（第2组， n = 70）和已解决（第3组， n = 33）HCV感染。在最近感染的患者中观察到的亲和力指数（AI）值显着低于在慢性携带者中发现的亲和力指数（12.0％±9.2％[平均值±标准差]）（83.1％±15.2％）。使用43.0％的阈值，该测定以非常高的灵敏度（98％）和特异性（100％）区分了第1组和第2组。对于第3组，观察到的AI值分布范围更广（54.8％±27.3％），表明该指数在HCV RNA阴性患者中无用。用一组来自17个HCV血清转化器的36个血清样品进行了试验的盲确认。此处描述的测定方法是区分HCV病毒血症患者近期感染和慢性感染的有用工具。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号