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首页> 外文期刊>Journal of Clinical Microbiology >Evaluation of Three Rapid Diagnostic Methods for Direct Identification of Microorganisms in Positive Blood Cultures
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Evaluation of Three Rapid Diagnostic Methods for Direct Identification of Microorganisms in Positive Blood Cultures

机译：直接鉴定阳性血液培养物中微生物的三种快速诊断方法的评价

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The identification of organisms from positive blood cultures generally takes several days. However, recently developed rapid diagnostic methods offer the potential for organism identification within only a few hours of blood culture positivity. In this study, we evaluated the performance of three commercial methods to rapidly identify organisms directly from positive blood cultures: QuickFISH (AdvanDx, Wolburn, MA), Verigene Gram-Positive Blood Culture (BC-GP; Nanosphere, Northbrook, IL), and matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) with Sepsityper processing (Bruker Daltonics, Billerica, MA). A total of 159 blood cultures (VersaTREK Trek Diagnostic Systems, Cleveland, OH) positive for Gram-positive and Gram-negative bacteria as well as yeast were analyzed with QuickFISH and MALDI-TOF MS. In all, 102 blood cultures were analyzed using the BC-GP assay. For monomicrobial cultures, we observed 98.0% concordance with routine methods for both QuickFISH (143/146) and the BC-GP assay (93/95). MALDI-TOF MS demonstrated 80.1% (117/146) and 87.7% (128/146) concordance with routine methods to the genus and species levels, respectively. None of the methods tested were capable of consistently identifying polymicrobial cultures in their entirety or reliably differentiating Streptococcus pneumoniae from viridans streptococci. Nevertheless, the methods evaluated in this study are convenient and accurate for the most commonly encountered pathogens and have the potential to dramatically reduce turnaround time for the provision of results to the treating physician.
机译：从阳性血液培养物中鉴定生物通常需要几天的时间。然而，最近开发的快速诊断方法提供了在血液培养阳性数小时内就可以鉴定出生物的潜力。在这项研究中，我们评估了三种可直接从阳性血液培养物中快速鉴定出生物的商业方法的性能： Quick FISH（AdvanDx，Wolburn，MA），Verigene革兰氏阳性血液培养（BC-GP;纳米球，伊利诺斯州诺斯布鲁克）和具有Sepsityper处理技术的基质辅助激光解吸电离飞行时间质谱仪（MALDI-TOF MS）（马萨诸塞州Billerica的布鲁克·道尔顿公司）。用 Quick FISH和MALDI-TOF MS分析了总共159种革兰氏阳性和革兰氏阴性细菌以及酵母阳性的血液培养物（VersaTREK Trek Diagnostic Systems，俄亥俄州克利夫兰）。总共使用BC-GP分析法分析了102种血液培养物。对于单微生物培养，我们观察到 Quick FISH（143/146）和BC-GP分析（93/95）的常规方法的一致性为98.0％。 MALDI-TOF MS与常规方法相比，其属和种水平分别显示80.1％（117/146）和87.7％（128/146）。所测试的方法均无法一致地整体鉴定微生物培养物或可靠地将肺炎链球菌与绿藻链球菌区分开。尽管如此，在这项研究中评估的方法对于最常见的病原体还是方便而准确的，并且有可能大大减少周转时间，从而为治疗医师提供结果。

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