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首页> 外文期刊>Journal of Clinical Microbiology >Simultaneous Detection of Immunoglobulin A (IgA) and IgM Antibodies against Hepatitis E Virus (HEV) Is Highly Specific for Diagnosis of Acute HEV Infection
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Simultaneous Detection of Immunoglobulin A (IgA) and IgM Antibodies against Hepatitis E Virus (HEV) Is Highly Specific for Diagnosis of Acute HEV Infection

机译：同时检测抗戊型肝炎病毒（HEV）的免疫球蛋白A（IgA）和IgM抗体对急性HEV感染的诊断具有高度特异性

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Serum samples collected from 68 patients (age, mean ± the standard deviation [SD], 56.3 ± 12.8 years) at admission who were subsequently molecularly diagnosed as having hepatitis E and from 2,781 individuals who were assumed not to have been recently infected with hepatitis E virus (HEV; negative controls; 52.9 ± 18.9 years), were tested for immunoglobulin M (IgM) and IgA classes of antibodies to HEV (anti-HEV) by in-house solid-phase enzyme immunoassay with recombinant open reading frame 2 protein expressed in the pupae of silkworm as the antigen probe. The 68 patients with hepatitis E had both anti-HEV IgM and anti-HEV IgA. Among the 2,781 controls, 16 (0.6%) had anti-HEV IgM alone and 4 (0.1%) had anti-HEV IgA alone: these IgA/IgM anti-HEV-positive individuals were not only negative for HEV RNA but lack IgG anti-HEV antibody as well (at least in most of the cases). Periodic serum samples obtained from 15 patients with hepatitis E were tested for HEV RNA, anti-HEV IgM, and anti-HEV IgA. Although HEV RNA was detectable in the serum until 7 to 40 (21.4 ± 9.7) days after disease onset, both IgM and IgA anti-HEV antibodies were detectable until 37, 55, or 62 days after disease onset in three patients and up through the end of the observation period (50 to 144 days) in 12 patients. These results indicate that detection of anti-HEV IgA alone or along with anti-HEV IgM is useful for serological diagnosis of hepatitis E with increased specificity and longer duration of positivity than that by RNA detection.
机译：从入院时被分子诊断为戊型肝炎的68名患者（年龄，平均值±标准差[SD]，56.3±12.8岁）收集血清样本，并从2781名被认为近期未感染戊型肝炎的个体中收集通过内部固相酶免疫测定法检测重组腺病毒（HEV;阴性对照; 52.9±18.9岁）的免疫球蛋白M（IgM）和IgA类抗HEV抗体（抗HEV），并表达重组开放阅读框2蛋白在家蚕the中作为抗原探针。 68例戊型肝炎患者同时患有抗HEV IgM和抗HEV IgA。在2,781名对照中，有16名（0.6％）仅抗HEV IgM，4名（0.1％）仅抗HEV IgA：这些IgA / IgM抗HEV阳性个体不仅对HEV RNA阴性，而且缺乏IgG抗-HEV抗体也是如此（至少在大多数情况下）。测试了从15名戊型肝炎患者中获得的定期血清样本的HEV RNA，抗HEV IgM和抗HEV IgA。尽管直到发病后7至40（21.4±9.7）天在血清中均可检测到HEV RNA，但三例患者直至发病后37、55或62天均可检测到IgM和IgA抗HEV抗体。 12例患者的观察期结束（50至144天）。这些结果表明，与RNA检测相比，单独检测抗HEV IgA或与抗HEV IgM一起检测戊型肝炎的血清学诊断具有更高的特异性和更长的阳性持续时间。

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